Further characterization of the binding of heparin to granulocyte colony-stimulating factor: Importance of sulfate groups
文献类型：期刊论文
| 作者 | Liang, AY; Liu, XJ; Du, YG; Wang, KY; Lin, BC |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2008 |
| 卷号 | 29期号:6页码:1286-1290 |
| 关键词 | capillary zone electrophoresis granulocyte colony-stimulating factor heparin interaction sulfate group |
| 通讯作者 | Lin, BC (reprint author), Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhonshan Rd, Dalian 116023, Peoples R China.,bclin@dicp.ac.cn |
| 英文摘要 | Heparin mediates fundamental biological mechanisms through interaction with proteins. Previously, we have shown that standard heparin binds to granulocyte colony-stimulating factor (G-CSF) with an affinity of 4.8 x 10(5) M-1. To further study the structural features in heparin that are responsible for this interaction, we studied the bindings of G-CSF and N-desulfated and 2,3-O-desulfated heparin by CZE. Results showed that the N-desulfated heparin had a similar affinity for G-CSF ((5.4 +/- 0.9) x 10(5) M-1), but the 2,3-O-desulfated heparin had a 1000-fold lower affinity ((3.4 +/- 1.2) x 10(2) M-1) in comparison to standard heparin. The results showed that 2,3-O-sulfate groups are more important than N-sulfate groups in heparin-G-CSF interaction. |
| 学科主题 | Biochemistry & Molecular Biology; Chemistry |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 关键词[WOS] | FIBROBLAST-GROWTH-FACTOR ; PERFORMANCE FRONTAL ANALYSIS ; HERPES-SIMPLEX-VIRUS ; AFFINITY CAPILLARY-ELECTROPHORESIS ; IN-VIVO ; RECEPTOR ; COMPLEX ; PROTEIN ; IDENTIFICATION ; PROTEOGLYCAN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000254882800010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1401]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liang, AY,Liu, XJ,Du, YG,et al. Further characterization of the binding of heparin to granulocyte colony-stimulating factor: Importance of sulfate groups[J]. ELECTROPHORESIS,2008,29(6):1286-1290. |
| APA | Liang, AY,Liu, XJ,Du, YG,Wang, KY,&Lin, BC.(2008).Further characterization of the binding of heparin to granulocyte colony-stimulating factor: Importance of sulfate groups.ELECTROPHORESIS,29(6),1286-1290. |
| MLA | Liang, AY,et al."Further characterization of the binding of heparin to granulocyte colony-stimulating factor: Importance of sulfate groups".ELECTROPHORESIS 29.6(2008):1286-1290. |
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