Characterization of the target DNA sequence for the DNA-binding domain of zinc finger protein 191
文献类型：期刊论文
| 作者 | Wang, HY; Sun, RL; Liu, GX; Yao, MH; Fei, J; Shen, HB |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2008 |
| 卷号 | 40期号:8页码:704-710 |
| 关键词 | zinc finger protein 191 DNA-binding protein microsatellite |
| 通讯作者 | Fei, J (reprint author), Tongji Univ, Sch Life Sci & Technol, Shanghai 200092, Peoples R China.,jfei@mail.tongji.edu.cn ; shenhb@shnu.edu.cn |
| 英文摘要 | Studies on the DNA-binding properties of transcription factors are important in searching for the downstream genes regulated by these factors. In the present study, we report on the DNA-binding property of a Cy s2His2-type transcription factor, zinc finger protein 191 (Zfp191), which has been newly found to play a significant role in mice. By constructing a fusion protein containingthe DNA-binding domain of Zfp191, we characterized target DNA by determining the protein's binding specificity and dependence on zinc. The data showed that the DNA-binding domain of Zfp191 can specifically bind to the TCAT repeat motif and that there is a cooperative effect amongthe target DNA's multiple binding sites. Furthermore, the binding reaction is dependent on zinc. This work provides a foundation for further studies on the role of Zfp191 in gene regulation and development. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | TYROSINE-HYDROXYLASE GENE ; CELL-GROWTH ; POLYMORPHIC MICROSATELLITE ; EMBRYONIC-DEVELOPMENT ; TRANSCRIPTION FACTORS ; EXPRESSION ; FAMILY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000258375800003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1409]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, HY,Sun, RL,Liu, GX,et al. Characterization of the target DNA sequence for the DNA-binding domain of zinc finger protein 191[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2008,40(8):704-710. |
| APA | Wang, HY,Sun, RL,Liu, GX,Yao, MH,Fei, J,&Shen, HB.(2008).Characterization of the target DNA sequence for the DNA-binding domain of zinc finger protein 191.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,40(8),704-710. |
| MLA | Wang, HY,et al."Characterization of the target DNA sequence for the DNA-binding domain of zinc finger protein 191".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 40.8(2008):704-710. |
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