Proteome identification of binding-partners interacting with cell polarity protein Par3 in Jurkat cells
文献类型：期刊论文
| 作者 | Zhou, Y; Fang, LH; Du, D; Zhou, WC; Feng, XJ; Chen, JW; Zhang, Z; Chen, ZJ |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2008 |
| 卷号 | 40期号:8页码:729-739 |
| 关键词 | cell polarity Par3 proteomics importin-alpha 4 PA28 beta PA28 gamma |
| 通讯作者 | Chen, ZJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,zjchen@sibs.ac.cn |
| 英文摘要 | The evolutionarily conserved cell polarity protein Par3, a scaffold-like PDZreontaining protein, plays a critical role in the establishment and maintenance of epithelial cell polarity. Although the role of Par3 in establishing cell polarity in epithelial cells has been intensively explored, the function of Par3 in hematopoietic cells remains elusive. To address this issue, we generated GST-fusion proteins of Par3 PDZ domains. By combiningthe GST-pull-down approach with liquid chromatography-tandem mass spectrometry, we identified 10 potential novel binding proteins of PDZ domains of Par3 in Jurkat cells (a T-cell line). The interaction of Par3 with three proteins-nuclear transport protein importin-alpha 4 and proteasome activators PA28 beta and PA28 gamma-was confirmed using in vitro binding assay, co-immunoprecipitation assay and immunofluorescence microscopy. Our results have the potential to uncover novel functions of the cell polarity protein Par3 in blood cells. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | TIGHT JUNCTION ; DROSOPHILA NEUROBLASTS ; PROTEASOME ACTIVATORS ; GLYCOLYTIC-ENZYMES ; ELEGANS EMBRYOS ; LOCALIZATION ; MIGRATION ; DNA ; PURIFICATION ; SEGREGATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000258375800006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1411]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhou, Y,Fang, LH,Du, D,et al. Proteome identification of binding-partners interacting with cell polarity protein Par3 in Jurkat cells[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2008,40(8):729-739. |
| APA | Zhou, Y.,Fang, LH.,Du, D.,Zhou, WC.,Feng, XJ.,...&Chen, ZJ.(2008).Proteome identification of binding-partners interacting with cell polarity protein Par3 in Jurkat cells.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,40(8),729-739. |
| MLA | Zhou, Y,et al."Proteome identification of binding-partners interacting with cell polarity protein Par3 in Jurkat cells".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 40.8(2008):729-739. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。