Identification and expression of GABA(C) receptor in rat testis and spermatozoa
文献类型：期刊论文
| 作者 | Li, SF; Zhang, YB; Liu, HX; Yan, YC; Li, YP |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2008 |
| 卷号 | 40期号:8页码:761-767 |
| 关键词 | GABA(C) receptor gamma-aminobutyric acid N(4)-chloroacetylcytosine arabinoside spermatozoa acrosome reaction |
| 通讯作者 | Li, YP (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,Grad Sch, Lab Mol Cell Biol,Shanghai Key Lab Mol Androl, Shanghai 200031, Peoples R China.,yipingli@sibs.ac.cn |
| 英文摘要 | Our previous studies showed that gamma-aminobutyric acid (GABA)(A) and GABA(B) receptors are involved in rat sperm acrosome reaction induced by progesterone or GABA. Here, we report the presence of GABA(C) receptor in rat testis and spermatozoa. Full-length complementary DNA encoding the rho 1, rho 2 and rho 3 subunits of GABA(C) receptor were cloned from rat testis; their sequences are identical to those of rat GABA(C) receptor in retina. Reverse transcription-polymerase chain reaction analysis showed that during the development of rat testis, the transcript levels of the rho 1 and rho 2 subunits showed little change, while the expression of rho 3 was gradually up-regulated. Immunofluorescence analysis using an anti-rho l antibody revealed that GABA(C) receptor exists on the elongated spermatid and sperm. Using a chlortetracycline assay, we found that N(4)-chloroacetylcytosine arabinoside, a GABA(C) receptor agonist, triggered rat sperm acrosome reaction; whereas (1,2,5,6-tetrahydropyridin-4-yl) methylphosphinic acid, a GABA(C) receptor antagonist, inhibited the ability of N(4)-chloroacetylcytosine arabinoside to induce acrosome reaction. These results suggested that GABA(C) receptors are also involved in rat sperm acrosome reaction. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | GAMMA-AMINOBUTYRIC-ACID ; ACROSOME REACTION ; MAMMALIAN SPERM ; SECRETING CELLS ; RHO-2 SUBUNITS ; ION CHANNELS ; BRAIN ; PROGESTERONE ; EXOCYTOSIS ; NEURONS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000258375800010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1413]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, SF,Zhang, YB,Liu, HX,et al. Identification and expression of GABA(C) receptor in rat testis and spermatozoa[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2008,40(8):761-767. |
| APA | Li, SF,Zhang, YB,Liu, HX,Yan, YC,&Li, YP.(2008).Identification and expression of GABA(C) receptor in rat testis and spermatozoa.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,40(8),761-767. |
| MLA | Li, SF,et al."Identification and expression of GABA(C) receptor in rat testis and spermatozoa".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 40.8(2008):761-767. |
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