Centrosome separation driven by actin-microfilaments during mitosis is mediated by centrosome-associated tyrosine-phosphorylated cortactin
文献类型：期刊论文
| 作者 | Wang, WQ; Chen, LY; Ding, YB; Jin, J; Liao, K |
| 刊名 | JOURNAL OF CELL SCIENCE
 |
| 出版日期 | 2008 |
| 卷号 | 121期号:8页码:1334-1343 |
| 关键词 | cortactin cortactin tyrosine phosphorylation centrosome separation actin-filament focal adhesion points Src kinase |
| 通讯作者 | Liao, K (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,kliao@sibs.ac.cn |
| 英文摘要 | The regulation of protein tyrosine phosphorylation is an important aspect during the cell cycle. From G2-M transition to mitotic anaphase, phosphorylation of Tyr421, Tyr466 and Tyr482 of cortactin, an actin-filament associated protein, is dramatically induced. The phosphorylated cortactin is almost exclusively associated with centrosomes or spindle poles during mitosis. At G2-M transition prior to the breakdown of the nuclear envelope, two duplicated centrosomes migrate towards opposite ends of the nucleus to form the spindle poles. This centrosome-separation process and also the start of mitosis are inhibited or delayed by the depolymerization of actin filaments. Also inhibited is the separation of centrosomes when a truncated form of cortactin is expressed, whose C-terminus contains the tyrosine phosphorylation region but lacks the actin-binding domains. We introduced mutations at the tyrosine phosphorylation sites in the truncated C-terminus of cortactin and found that the C-terminus could no longer interfere with centrosome separation process. Our study shows that, cortactin phosphorylated at Tyr421, Tyr466 and Tyr482 mediates the actin-filament-driven centrosome separation at G2-M transition by providing a bridge between the centrosome and actin-filaments. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | SRC OVEREXPRESSER CELLS ; C-SRC ; INTERPHASE CELLS ; PROTEIN ; CYTOSKELETON ; KINASE ; CYCLE ; VERTEBRATES ; ACTIVATION ; INDUCTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000254660500021 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1420]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, WQ,Chen, LY,Ding, YB,et al. Centrosome separation driven by actin-microfilaments during mitosis is mediated by centrosome-associated tyrosine-phosphorylated cortactin[J]. JOURNAL OF CELL SCIENCE,2008,121(8):1334-1343. |
| APA | Wang, WQ,Chen, LY,Ding, YB,Jin, J,&Liao, K.(2008).Centrosome separation driven by actin-microfilaments during mitosis is mediated by centrosome-associated tyrosine-phosphorylated cortactin.JOURNAL OF CELL SCIENCE,121(8),1334-1343. |
| MLA | Wang, WQ,et al."Centrosome separation driven by actin-microfilaments during mitosis is mediated by centrosome-associated tyrosine-phosphorylated cortactin".JOURNAL OF CELL SCIENCE 121.8(2008):1334-1343. |
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