In vitro observation of the molecular interaction between NodD and its inducer naringenin as monitored by fluorescence resonance energy transfer
文献类型：期刊论文
| 作者 | Li, FQ; Hou, BH; Chen, L; Yao, ZJ; Hong, GF |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2008 |
| 卷号 | 40期号:9页码:783-789 |
| 关键词 | NodD naringenin fluorescence resonance energy transfer (FRET) |
| 通讯作者 | Hong, GF (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,gfhong@sibs.ac.cn |
| 英文摘要 | At initial stages in the Rhizobium legume symbiosis, most Modulation genes are controlled by NodD protein and plant inducers. Some genetic studies and other reports have suggested that NodD may be activated by its direct interaction with plant inducers. However, there has been no molecular evidence of such an inducing interaction. In this paper, we used fluorescence resonance energy transfer technique to see whether such an interaction exists between NodD and its activator, naringenin, in vitro. The tetracysteine motif (Cys-Cys-Pro-Gly-Cys-Cys) was genetically inserted into NodD to label NodD with 4',5'-bis(1,3,2-dithioarsolan-2-yl) fluorescein (FlAsH). Naringenin was labeled with fluorescein by chemical linking. In the fluorescence resonance energy transfer experiments in vitro, the fluorescence intensity of one acceptor, NodD(90R6)-FlAsH, increased by 13%. This suggests that NodD may directly interact with inducer naringenin in vitro and that the reaction centre is likely near hinge region 1 of NodD. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | BROAD HOST RANGE ; LEGUMINOSARUM BIOVAR VICIAE ; RHIZOBIUM-LEGUMINOSARUM ; NODULATION GENES ; PRODUCT ; EXPRESSION ; MOTIFS ; FRET ; LOCALIZATION ; MUTAGENESIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000259676100003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1424]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, FQ,Hou, BH,Chen, L,et al. In vitro observation of the molecular interaction between NodD and its inducer naringenin as monitored by fluorescence resonance energy transfer[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2008,40(9):783-789. |
| APA | Li, FQ,Hou, BH,Chen, L,Yao, ZJ,&Hong, GF.(2008).In vitro observation of the molecular interaction between NodD and its inducer naringenin as monitored by fluorescence resonance energy transfer.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,40(9),783-789. |
| MLA | Li, FQ,et al."In vitro observation of the molecular interaction between NodD and its inducer naringenin as monitored by fluorescence resonance energy transfer".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 40.9(2008):783-789. |
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