RNA secondary structures located in the interchromosomal region of human ACAT1 chimeric mRNA are required to produce the 56-kDa isoform
文献类型：期刊论文
| 作者 | Chen, J; Zhao, XN; Yang, L; Hu, GJ; Lu, M; Xiong, Y; Yang, XY; Chang, CCY; Song, BL; Chang, TY |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2008 |
| 卷号 | 18期号:9页码:921-936 |
| 关键词 | human ACAT1 isoform chimeric human ACAT1 mRNA interchromosomal region RNA secondary structure internal ribosome entry site |
| 通讯作者 | Li, BL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,blli@sibs.ac.cn |
| 英文摘要 | processing of two discontinuous RNAs transcribed from chromosomes 1 and 7. The chimeric mRNA uses AUG(1397-1399) and GGC(1274-1276) as translation initiation codons to produce normal 50-kDa ACAT1 and a novel enzymatically active 56-kDa isoform, respectively, with the latter being authentically present in human cells, including human monocyte-derived macrophages. In this work, we report that RNA secondary structures located in the vicinity of the GGC(1274-1276) codon are required for production of the 56-kDa isoform. The effects of the three predicted stem-loops (nt 1255-1268, 1286-1342 and 1355-1384) were tested individually by transfecting expression plasmids into cells that contained the wild-type, deleted or mutant stem-loop sequences linked to a partial ACAT1 AUG open reading frame (ORF) or to the ORFs of other genes. The expression patterns were monitored by western blot analyses. We found that the upstream stem-loop(1255-1268) from chromosome 7 and downstream stem-loop(1286-1342) from chromosome 1 were needed for production of the 56-kDa isoform, whereas the last stem-loop(1355-1384) from chromosome 1 was dispensable. The results of experiments using both monocistronic and bicistronic vectors with a stable hairpin showed that translation initiation from the GGC(1274-1276) codon was mediated by an internal ribosome entry site (IRES). Further experiments revealed that translation initiation from the GGC(1274-1276) codon requires the upstream AU-constituted RNA secondary structure and the downstream GC-rich structure. This mechanistic work provides further support for the biological significance of the chimeric nature of the human ACAT1 transcript. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | A-CHOLESTEROL ACYLTRANSFERASE ; INTERNAL RIBOSOMAL ENTRY ; 2 DIFFERENT CHROMOSOMES ; ACYL-COENZYME ; TRANSLATION INITIATION ; BINDING-PROTEIN ; GENE-EXPRESSION ; HEPATITIS-C ; IN-VITRO ; VIRUS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000259891200004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1428]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, J,Zhao, XN,Yang, L,et al. RNA secondary structures located in the interchromosomal region of human ACAT1 chimeric mRNA are required to produce the 56-kDa isoform[J]. CELL RESEARCH,2008,18(9):921-936. |
| APA | Chen, J.,Zhao, XN.,Yang, L.,Hu, GJ.,Lu, M.,...&Li, BL.(2008).RNA secondary structures located in the interchromosomal region of human ACAT1 chimeric mRNA are required to produce the 56-kDa isoform.CELL RESEARCH,18(9),921-936. |
| MLA | Chen, J,et al."RNA secondary structures located in the interchromosomal region of human ACAT1 chimeric mRNA are required to produce the 56-kDa isoform".CELL RESEARCH 18.9(2008):921-936. |
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