Significant antitumor activity of oncolytic adenovirus expressing human interferon-beta for hepatocellular carcinoma
文献类型：期刊论文
| 作者 | He, LF; Gu, JF; Tang, WH; Fan, JK; Wei, N; Zou, WG; Zhang, YH; Zhao, LL; Liu, XY |
| 刊名 | JOURNAL OF GENE MEDICINE
 |
| 出版日期 | 2008 |
| 卷号 | 10期号:9页码:983-992 |
| 关键词 | oncolytic adenovirus interferon-beta targeting gene-virotherapy hepatocellular carcinoma |
| 通讯作者 | Liu, XY (reprint author), Chinese Acad Sci, Lab Canc Therapy, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,xyliu@sibs.ac.cn |
| 英文摘要 | Background Human interferon-beta (IFN-beta) has been widely used in gene therapy for its antitumor activity but its therapeutic effect is limited. The conditionally replicative adenovirus ONYX-015 (a E1B-55-kDa-deleted adenovirus) targets well to tumor cells, but is not potent enough to cause significant tumor regression. To solve these problems, a tumor-selective replicating adenovirus expressing IFN-beta was constructed in this study. Methods The oncolytic adenoviruses were generated by homologous recombination in packaging cells. The expression of the IFN-beta protein was detected by enzyme-linked immunosorbent assay (ELISA). The antitumor efficacy of ZD55-IFN-beta was evaluated in cell lines and human hepatocellular carcinoma xenografts in nude mice. Results ZD55-IFN-beta can express much more IFN-beta than Ad-IFN-beta because of the replication of the ZD55 vector. Our data showed that ZD55-IFN-beta could exert a strong cytopathic effect on tumor cells (about 100-fold higher than Ad-IFN-beta or ONYX-015). Moreover, no obvious cytotoxic or apoptotic effects were detected in normal cells infected with ZD55-IFN-beta. Conclusions The antitumor efficacy of IFN-beta could be significantly improved due to the increased gene expression level from the tumor-selective replicating vector. The oncolytic adenovirus expressing IFN-beta may provide a novel approach for cancer gene therapy. Copyright (C) 2008 John Wiley & Sons, Ltd. |
| 学科主题 | Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental |
| 关键词[WOS] | APOPTOSIS-INDUCING LIGAND ; TARGETING GENE-VIROTHERAPY ; REPLICATING ADENOVIRUS ; CANCER-CELLS ; NECK-CANCER ; TUMOR ; THERAPY ; ONYX-015 ; TRIAL ; REGRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000260361600004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1429]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | He, LF,Gu, JF,Tang, WH,et al. Significant antitumor activity of oncolytic adenovirus expressing human interferon-beta for hepatocellular carcinoma[J]. JOURNAL OF GENE MEDICINE,2008,10(9):983-992. |
| APA | He, LF.,Gu, JF.,Tang, WH.,Fan, JK.,Wei, N.,...&Liu, XY.(2008).Significant antitumor activity of oncolytic adenovirus expressing human interferon-beta for hepatocellular carcinoma.JOURNAL OF GENE MEDICINE,10(9),983-992. |
| MLA | He, LF,et al."Significant antitumor activity of oncolytic adenovirus expressing human interferon-beta for hepatocellular carcinoma".JOURNAL OF GENE MEDICINE 10.9(2008):983-992. |
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