Equilibrium folding of porcine insulin precursor in the presence of redox buffer: Implications for the common intermediates shared by its unfolding/refolding processes
文献类型：期刊论文
| 作者 | Zhao, J; Huang, QL; Tang, YH; Guo, ZY; Qiao, ZS; Xu, GJ; Feng, YM |
| 刊名 | PROTEIN AND PEPTIDE LETTERS
 |
| 出版日期 | 2008 |
| 卷号 | 15期号:9页码:972-979 |
| 关键词 | insulin single-chain insulin folding unfolding intermediate disulfide |
| 通讯作者 | Feng, YM (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, SIBS, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,fengym@sunm.shcnc.ac.cn |
| 英文摘要 | We use the procedure established for 'disulfide stability analysis in redox system' to investigate the unfolding process of porcine insulin precursor (PIP). Six major unfolding intermediates have been captured, in which four contain two disulfides, two contain one disulfide. Based on the characterization and analysis of the intermediates an unfolding pathway has been proposed, by which the native PIP unfolded through in turn 2SS and 1SS intermediates into fully reduced form. Besides, the comparison of the intermediates captured in PIP unfolding process with those intermediates captured in its refolding process revealed that some intermediates captured during both unfolding/refolding processes of PIP have identical disulfide pairing pattern, from which we suggest that the unfolding/refolding processes of PIP share some common intermediates but flow in the opposite direction. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | BOVINE PANCREATIC RIBONUCLEASE ; DISULFIDE-FORMING PATHWAY ; SINGLE-CHAIN INSULIN ; GROWTH-FACTOR-I ; BOND FORMATION ; PEPTIDE MODEL ; PROTEIN ; VITRO ; IDENTIFICATION ; REGENERATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000259509800016 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1434]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, J,Huang, QL,Tang, YH,et al. Equilibrium folding of porcine insulin precursor in the presence of redox buffer: Implications for the common intermediates shared by its unfolding/refolding processes[J]. PROTEIN AND PEPTIDE LETTERS,2008,15(9):972-979. |
| APA | Zhao, J.,Huang, QL.,Tang, YH.,Guo, ZY.,Qiao, ZS.,...&Feng, YM.(2008).Equilibrium folding of porcine insulin precursor in the presence of redox buffer: Implications for the common intermediates shared by its unfolding/refolding processes.PROTEIN AND PEPTIDE LETTERS,15(9),972-979. |
| MLA | Zhao, J,et al."Equilibrium folding of porcine insulin precursor in the presence of redox buffer: Implications for the common intermediates shared by its unfolding/refolding processes".PROTEIN AND PEPTIDE LETTERS 15.9(2008):972-979. |
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