Neuregulin-1 promotes cardiomyocyte differentiation of genetically engineered embryonic stem cell clones
文献类型：期刊论文
| 作者 | Wang, Z; Xu, GT; Wu, YL; Liu, SW; Sun, BG; Dai, QY |
| 刊名 | BMB REPORTS
 |
| 出版日期 | 2008 |
| 卷号 | 41期号:10页码:699-704 |
| 关键词 | Cardiomyocytes Embryonic stem cells Enhanced green fluorescent protein Neuregulin-1 |
| 通讯作者 | Dai, QY (reprint author), Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 1, Dept Cardiol, Shanghai 200030, Peoples R China.,qiuyand@yahoo.com |
| 英文摘要 | Embryonic stem (ES) cell-derived cardiomyocytes (ESCMs) must be specifically purified in order to prevent teratoma formation, and this confusing issue has hampered their clinical application. We therefore investigated a technique to generate pure labeled ESCMs for possible use in cardiac repair. We generated transgenic ES cell lines expressing enhanced green fluorescent protein (EGFP) under the transcriptional control of the a-cardiac myosin heavy chain (alpha-MHC) promoter. Differentiated EGFP-positive ES cells displayed characteristics of CMs. Furthermore, neuregulin-1 (NRG-1) upregulated the expression of the cardiac-restricted transcription factors Nkx2.5 and GATA-4, as well as differentiated CM factors (alpha-MHC, beta-MHC). Immunohistochemistry demonstrated that NRG-1 increased expression of cardiac-specific troponin T in the beating foci of the embryoid bodies. This work revealed a potential method for specifically labeling and enriching ESCMs by combining genetically-engineered ES cell clones and exogenous growth factor treatment. [BMB reports 2008, 41(10): 699-704] |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | CARDIAC PRECURSOR CELLS ; IN-VITRO ; ES CELLS ; HEART ; ACTIVATION ; EXPRESSION ; MYOCYTES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000260569500003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1439]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Z,Xu, GT,Wu, YL,et al. Neuregulin-1 promotes cardiomyocyte differentiation of genetically engineered embryonic stem cell clones[J]. BMB REPORTS,2008,41(10):699-704. |
| APA | Wang, Z,Xu, GT,Wu, YL,Liu, SW,Sun, BG,&Dai, QY.(2008).Neuregulin-1 promotes cardiomyocyte differentiation of genetically engineered embryonic stem cell clones.BMB REPORTS,41(10),699-704. |
| MLA | Wang, Z,et al."Neuregulin-1 promotes cardiomyocyte differentiation of genetically engineered embryonic stem cell clones".BMB REPORTS 41.10(2008):699-704. |
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