New member of the guanosine triphosphatase activating protein family in the human epididymis
文献类型：期刊论文
| 作者 | Li, XQ; Liu, Q; Liu, SG; Zhang, JS; Zhang, YL |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2008 |
| 卷号 | 40期号:10页码:855-863 |
| 关键词 | epididymis MacGAP RhoGAP signal transduction |
| 通讯作者 | Zhang, YL (reprint author), Chinese Acad Sci, Shanghai Key Lab Mol Androl, Inst Biochem & Cell Biol, State Key Lab Mol Biol,Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,ylzhang@sibs.ac.cn |
| 英文摘要 | The effect of the guanosine triphosphatase activating proteins (GAPs) on spermatogenesis has been studied for years, though no GAPs have been explored in epididymis, an essential organ for sperm maturation. In this study, a new GAP member, designated as MacGAP, was cloned in human epididymis. The Mac GAP geneencodesaprotein of 618 amino acids with a putative size of 70 kDa and harbors the conserved RhoGAP domain. The N-terminal and C-terminal peptides of MacGAP were expressed and their corresponding antisera were prepared. The antisera against N-terminal peptide could detect antigen as low as 0.3 ng, and its specificity was also confirmed. However, the antisera against C-terminal peptide failed to detect its antigen because of its low sensitivity. Immunohistochemistry showed that the MacGAP protein was dependent on epididymis and had a region-specific expression pattern, with high expression in the epithelial cells' basal section in the caput region. The results have created a foundation for further interpretation of the biological effects of GAPs in sperm maturation. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | POLYCLONAL ANTISERA ; SPERM MATURATION ; BETA-DEFENSIN ; EXPRESSION ; DROSOPHILA ; SPERMATOGENESIS ; IDENTIFICATION ; CAPACITATION ; FERTILITY ; ESP13.2 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000260131100003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1441]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, XQ,Liu, Q,Liu, SG,et al. New member of the guanosine triphosphatase activating protein family in the human epididymis[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2008,40(10):855-863. |
| APA | Li, XQ,Liu, Q,Liu, SG,Zhang, JS,&Zhang, YL.(2008).New member of the guanosine triphosphatase activating protein family in the human epididymis.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,40(10),855-863. |
| MLA | Li, XQ,et al."New member of the guanosine triphosphatase activating protein family in the human epididymis".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 40.10(2008):855-863. |
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