Fractionation of complex protein mixture by virtual three-dimensional liquid chromatography based on combined pH and salt steps
文献类型：期刊论文
| 作者 | Ning, ZB; Li, QR; Dai, J; Li, RX; Shieh, CH; Zeng, R |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2008 |
| 卷号 | 7期号:10页码:4525-4537 |
| 关键词 | virtual three-dimensional chromatography liquid chromatography pH elution combined mode plasma |
| 通讯作者 | Zeng, R (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Syst Biol, Shanghai 200031, Peoples R China.,zr@sibs.ac.cn |
| 英文摘要 | The complexity and diversity of biological samples in proteomics require intensive fractionation ahead of mass spectrometry identification. This work developed a chromatographic method called virtual three-dimensional chromatography to fractionate complex protein mixtures. By alternate elution with different pHs and salt concentrations, we implemented pH and salt steps by turns on a single strong cation exchange column to fully exploit its chromatographic ability.,Given standard proteins that were not resolved solely by pH or salt gradient elution could be successfully separated using this combined mode. With a reversed phase column tandem connected behind, we further fractionated as well as desalted proteins as the third dimension. This present strategy could readily be adapted with respect to special complexity of biological samples. Crude plasma without depleting high abundance proteins were fractionated by this three-dimensional mode and then analyzed by reversed phase liquid chromatography coupled with LTQ mass spectrometry. In total, 1933 protein groups with wide dynamic ranges were identified from a single experiment. Some characteristics that correlated to the behavior of proteins on strong cation exchange columns are also discussed. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemical Research Methods |
| 关键词[WOS] | TANDEM MASS-SPECTROMETRY ; HUMAN PLASMA PROTEOME ; DYNAMIC-RANGE CHARACTERIZATION ; ANION-EXCHANGE CHROMATOGRAPHY ; HIGH-ABUNDANT PROTEINS ; HUMAN SERUM PROTEOME ; HUMAN BLOOD-PLASMA ; MULTIDIMENSIONAL CHROMATOGRAPHY ; 2-DIMENSIONAL ELECTROPHORESIS ; CAPILLARY-ELECTROPHORESIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000259784300031 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1444]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ning, ZB,Li, QR,Dai, J,et al. Fractionation of complex protein mixture by virtual three-dimensional liquid chromatography based on combined pH and salt steps[J]. JOURNAL OF PROTEOME RESEARCH,2008,7(10):4525-4537. |
| APA | Ning, ZB,Li, QR,Dai, J,Li, RX,Shieh, CH,&Zeng, R.(2008).Fractionation of complex protein mixture by virtual three-dimensional liquid chromatography based on combined pH and salt steps.JOURNAL OF PROTEOME RESEARCH,7(10),4525-4537. |
| MLA | Ning, ZB,et al."Fractionation of complex protein mixture by virtual three-dimensional liquid chromatography based on combined pH and salt steps".JOURNAL OF PROTEOME RESEARCH 7.10(2008):4525-4537. |
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