Distinct subcellular distribution of delta-opioid receptor fused with various tags in PC12 cells
文献类型：期刊论文
| 作者 | Wang, HB; Guan, JS; Bao, L; Zhang, X |
| 刊名 | NEUROCHEMICAL RESEARCH
 |
| 出版日期 | 2008 |
| 卷号 | 33期号:10页码:2028-2034 |
| 关键词 | delta-Opioid receptor tag-fused receptor large dense-core vesicle subcellular distribution |
| 通讯作者 | Zhang, X (reprint author), Chinese Acad Sci, Inst Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,xu.zhang@ion.ac.cn |
| 英文摘要 | In small dorsal root ganglion neurons, delta-opioid receptors (DORs) have been found to be mainly distributed in the cytoplasm and often associated with the membrane of large dense-core vesicles (LDCVs) that contain neuropeptides. To study the distribution of DORs under various physiological or pharmacological conditions, the receptors fused with different tags are constructed, transfected into cells or animals, and examined with microscopy. In this study, we show that DOR with different tags have distinct patterns of subcellular distribution in neuroendocrine cells, PC12 cells. Both immunostaining and vesicle fraction analysis showed that the native DORs expressed in PC12 cells were mainly associated with LDCVs. In transfected PC12 cells, DOR tagged with Myc or hemagglutinin exhibited LDCV localization. However, DOR fused with GFP at N- or C-terminus was found to be mainly localized on the cell surface, and mediated the function of DOR agonist. Therefore, the distribution of DOR fused with GFP differs from the native DORs. These results suggest that the subcellular distribution of the receptor could be better presented by the fused tag with smaller molecular size. |
| 学科主题 | Biochemistry & Molecular Biology; Neurosciences & Neurology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Neurosciences |
| 关键词[WOS] | PLASMA-MEMBRANE ; GLYCINE RECEPTOR ; PICHIA-PASTORIS ; SPINAL-CORD ; PROTEIN ; EXPRESSION ; TRAFFICKING ; AGONIST ; RAT ; LOCALIZATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000259190900016 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1446]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, HB,Guan, JS,Bao, L,et al. Distinct subcellular distribution of delta-opioid receptor fused with various tags in PC12 cells[J]. NEUROCHEMICAL RESEARCH,2008,33(10):2028-2034. |
| APA | Wang, HB,Guan, JS,Bao, L,&Zhang, X.(2008).Distinct subcellular distribution of delta-opioid receptor fused with various tags in PC12 cells.NEUROCHEMICAL RESEARCH,33(10),2028-2034. |
| MLA | Wang, HB,et al."Distinct subcellular distribution of delta-opioid receptor fused with various tags in PC12 cells".NEUROCHEMICAL RESEARCH 33.10(2008):2028-2034. |
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