Structure-function relationship of bifunctional scorpion toxin BmBKTx1
文献类型：期刊论文
| 作者 | Wang, SM; Huang, LJ; Wicher, D; Chi, CW; Xu, CQ |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2008 |
| 卷号 | 40期号:11页码:955-963 |
| 关键词 | BmBKTx1 scorpion K(+) channel toxin (KTx) K(+) channel two functional faces |
| 通讯作者 | Chi, CW (reprint author), Tongji Univ, Coll Life Sci & Technol, Inst Prot Res, Shanghai 200092, Peoples R China.,zwqi@sibs.ac.cn ; Chenqi_xu@dfci.harvard.edu |
| 英文摘要 | As the first identified scorpion toxin active on both big conductance Ca(2+)-activated K(+) channels (BK) and small c on duc t a n c e Ca(2+)-activated K(+) channels (SK), BmBKTx1 has been proposed to have two separate functional faces for two targets. To investigate this hypothesis, two double mutants, K21A-Y30A and R9A-K11A, together with wild-type toxin were expressed in Escherichia coli. The recombinant toxins were tested on cockroach BK and rat SK2 channel for functional assay. Mutant K21A-Y30A had a dramatic loss of function on BK but retained its function on SK. Mutant R9A-K11A did not lose function on BK or SK. These data support the two functional-face hypothesis and indicate that the BK face is on the C-terminal beta-sheet. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | BUTHUS-MARTENSI KARSCH ; POTASSIUM CHANNEL ; K+ CHANNELS ; CONVERGENT EVOLUTION ; MOLECULAR-BASIS ; ANIMAL TOXINS ; RECOGNITION ; SELECTIVITY ; BLOCKERS ; CURRENTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000261324400007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1452]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, SM,Huang, LJ,Wicher, D,et al. Structure-function relationship of bifunctional scorpion toxin BmBKTx1[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2008,40(11):955-963. |
| APA | Wang, SM,Huang, LJ,Wicher, D,Chi, CW,&Xu, CQ.(2008).Structure-function relationship of bifunctional scorpion toxin BmBKTx1.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,40(11),955-963. |
| MLA | Wang, SM,et al."Structure-function relationship of bifunctional scorpion toxin BmBKTx1".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 40.11(2008):955-963. |
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