Yamanaka factors critically regulate the developmental signaling network in mouse embryonic stem cells
文献类型：期刊论文
| 作者 | Liu, XS; Huang, JY; Chen, T; Wang, Y; Xin, SM; Li, J; Pei, G; Kang, JH |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2008 |
| 卷号 | 18期号:12页码:1177-1189 |
| 关键词 | embryonic stem cell pluripotency Yamanaka factor signal pathway ChIP-on-chip |
| 通讯作者 | Kang, JH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China.,jhkang@sibs.ac.cn |
| 英文摘要 | Yamanaka factors (Oct3/4, Sox2, Klf4, c-Myc) are highly expressed in embryonic stem (ES) cells, and their overexpression can induce pluripotency in both mouse and human somatic cells, indicating that these factors regulate the developmental signaling network necessary for ES cell pluripotency. However, systemic analysis of the signaling pathways regulated by Yamanaka factors has not yet been fully described. In this study, we identified the target promoters of endogenous Yamanaka factors on a whole genome scale using ChIP ( chromatin immunoprecipitation)-on-chip in E14.1 mouse ES cells, and we found that these four factors co-occupied 58 promoters. Interestingly, when Oct4 and Sox2 were analyzed as core factors, Klf4 functioned to enhance the core factors for development regulation, whereas c-Myc seemed to play a distinct role in regulating metabolism. The pathway analysis revealed that Yamanaka factors collectively regulate a developmental signaling network composed of 16 developmental signaling pathways, nine of which represent earlier unknown pathways in ES cells, including apoptosis and cell-cycle pathways. We further analyzed data from a recent study examining Yamanaka factors in mouse ES cells. Interestingly, this analysis also revealed 16 developmental signaling pathways, of which 14 pathways overlap with the ones revealed by this study, despite that the target genes and the signaling pathways regulated by each individual Yamanaka factor differ significantly between these two datasets. We suggest that Yamanaka factors critically regulate a developmental signaling network composed of approximately a dozen crucial developmental signaling pathways to maintain the pluripotency of ES cells and probably also to induce pluripotent stem cells. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | TRANSCRIPTIONAL REGULATION ; TGF-BETA ; HUMAN FIBROBLASTS ; FAMILY PROTEINS ; CANCER-THERAPY ; BINDING-SITE ; MYC TARGET ; CROSS-TALK ; ES CELLS ; WNT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000261817900004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1460]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, XS,Huang, JY,Chen, T,et al. Yamanaka factors critically regulate the developmental signaling network in mouse embryonic stem cells[J]. CELL RESEARCH,2008,18(12):1177-1189. |
| APA | Liu, XS.,Huang, JY.,Chen, T.,Wang, Y.,Xin, SM.,...&Kang, JH.(2008).Yamanaka factors critically regulate the developmental signaling network in mouse embryonic stem cells.CELL RESEARCH,18(12),1177-1189. |
| MLA | Liu, XS,et al."Yamanaka factors critically regulate the developmental signaling network in mouse embryonic stem cells".CELL RESEARCH 18.12(2008):1177-1189. |
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