Preparation, Characterization and Application of High Specific Polyclonal Antibody Against Pancreatic Lipase Related Protein 1
文献类型：期刊论文
| 作者 | Jian-Ke, R; Jia-Juan, S; Zhe-Jin, S; Jian, F |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2008 |
| 卷号 | 35期号:12页码:1410-1416 |
| 关键词 | specificity pancreatic lipase related protein 1 (PLRP1) polyclonal antibody GST fusion protein |
| 通讯作者 | Jian, F (reprint author), Shanghai Nan Fang Model Orgnasim Res Ctr, Shanghai 201203, Peoples R China.,jfei@mail.tongji.edu.cn |
| 英文摘要 | Pancreatic acinar cells synthesize pancreatic lipase related protein 1 (PLRP1), which has a high degree of sequence and structural homology with pancreatic triglyceride lipase (PTL). PTL is required for efficient dietary triglyceride digestion, while the physiological role of PLRP1 has not been elucidated, although some investigations have shown that its expression level is changed under some physiological or pathological conditions. Specific antigenic peptides were fused to glutathione S-transferase (GST) and purified recombinant fusion protein was used to generate polyclonal antisera by immunization of rabbits. The antisera could detect the antigen as low as 0.6 ng and PLRP1 protein in 3 mu g of mouse pancreatic juice extracts. The high specificity was verified in Western blot and immunohistochemistry analysis by using PLRP1 knockout (KO) mice as the control. Furthermore, it was showed that food intake could increase the exocrine secretion of PLRP1 into pancreatic juice. This implied that PLRP1 may fulfill dietary digestion function in the digestion track. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | KINETIC-PROPERTIES ; MESSENGER-RNA ; IDENTIFICATION ; COLIPASE ; GENE ; GP-3 ; MEMBRANE ; CLONING ; MOUSE ; JUICE |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000262041400010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1461]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Jian-Ke, R,Jia-Juan, S,Zhe-Jin, S,et al. Preparation, Characterization and Application of High Specific Polyclonal Antibody Against Pancreatic Lipase Related Protein 1[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2008,35(12):1410-1416. |
| APA | Jian-Ke, R,Jia-Juan, S,Zhe-Jin, S,&Jian, F.(2008).Preparation, Characterization and Application of High Specific Polyclonal Antibody Against Pancreatic Lipase Related Protein 1.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,35(12),1410-1416. |
| MLA | Jian-Ke, R,et al."Preparation, Characterization and Application of High Specific Polyclonal Antibody Against Pancreatic Lipase Related Protein 1".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 35.12(2008):1410-1416. |
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