Different roles for two ubiquitin-like domains of ISG15 in protein modification
文献类型：期刊论文
| 作者 | Chang, YG; Yan, XZ; Xie, YY; Gao, XC; Song, AX; Zhang, DE; Hu, HY |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2008 |
| 卷号 | 283期号:19页码:13370-13377 |
| 通讯作者 | Hu, HY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,hyhu@sibs.ac.cn |
| 英文摘要 | ISG15 (interferon-stimulated gene 15) is a novel ubiquitin-like (UbL) modifier with two UbL domains in its architecture. We investigated different roles for the two UbL domains in protein modification by ISG15 (ISGylation) and the impact of Influenza B virus NS1 protein (NS1B) on regulation of the pathway. The results show that, although the C-terminal domain is sufficient to link ISG15 to UBE1L and UbcH8, the N-terminal domain is dispensable in the activation and transthiolation steps but required for efficient E3-mediated transfer of ISG15 from UbcH8 to its substrates. NS1B specifically binds to the N-terminal domain of ISG15 but does not affect ISG15 linkage via a thioester bond to its activating and conjugating enzymes. However, it does inhibit the formation of cellular ISG15 conjugates upon interferon treatment. We propose that the N-terminal UbL domain of ISG15 mainly functions in the ligation step and NS1B inhibits ISGylation by competing with E3 ligases for binding to the N-terminal domain. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | ANTIVIRAL MOLECULE ; STIMULATED GENE-15 ; HUMAN-CELLS ; E3 LIGASE ; INTERFERON ; CONJUGATION ; INFLUENZA ; TARGETS ; ENZYME ; E2 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000255499800076 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1471]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chang, YG,Yan, XZ,Xie, YY,et al. Different roles for two ubiquitin-like domains of ISG15 in protein modification[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2008,283(19):13370-13377. |
| APA | Chang, YG.,Yan, XZ.,Xie, YY.,Gao, XC.,Song, AX.,...&Hu, HY.(2008).Different roles for two ubiquitin-like domains of ISG15 in protein modification.JOURNAL OF BIOLOGICAL CHEMISTRY,283(19),13370-13377. |
| MLA | Chang, YG,et al."Different roles for two ubiquitin-like domains of ISG15 in protein modification".JOURNAL OF BIOLOGICAL CHEMISTRY 283.19(2008):13370-13377. |
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