Functional imaging of interleukin 1 beta expression in inflammatory process using bioluminescence imaging in transgenic mice
文献类型：期刊论文
| 作者 | Li, LM; Fei, ZL; Ren, JK; Sun, RL; Liu, ZH; Sheng, ZJ; Wang, L; Sun, X; Yu, J; Wang, ZG |
| 刊名 | BMC IMMUNOLOGY
 |
| 出版日期 | 2008 |
| 卷号 | 9期号:1页码:49-49 |
| 通讯作者 | Fei, J (reprint author), Shanghai Res Ctr Model Organisms, Pu Dong, Shanghai, Peoples R China.,lmli@sibs.ac.cn ; s123d@126.com ; jkren@sibs.ac.cn ; rlsun@sibs.ac.cn ; zhliu@sibs.ac.cn ; szj120@yahoo.com.cn ; lwang@126.com ; xsun@126.com ; jyu@shanghaigenomics.com ; zgangwang@126.com ; jfei@mail.tongji.edu.cn |
| 英文摘要 | Background: Interleukin 1 beta ( IL-1 beta) plays an important role in a number of chronic and acute inflammatory diseases. To understand the role of IL-1 beta in disease processes and develop an in vivo screening system for anti-inflammatory drugs, a transgenic mouse line was generated which incorporated the transgene firefly luciferase gene driven by a 4.5-kb fragment of the human IL-1 beta gene promoter. Luciferase gene expression was monitored in live mice under anesthesia using bioluminescence imaging in a number of inflammatory disease models. Results: In a LPS-induced sepsis model, dramatic increase in luciferase activity was observed in the mice. This transgene induction was time dependent and correlated with an increase of endogenous IL-1 beta mRNA and pro-IL-1 beta protein levels in the mice. In a zymosan-induced arthritis model and an oxazolone-induced skin hypersensitivity reaction model, luciferase expression was locally induced in the zymosan injected knee joint and in the ear with oxazolone application, respectively. Dexamethasone suppressed the expression of luciferase gene both in the acute sepsis model and in the acute arthritis model. Conclusion: Our data suggest that the transgenic mice model could be used to study transcriptional regulation of the IL-1 beta gene expression in the inflammatory process and evaluation the effect of anti-inflammatory drug in vivo. |
| 学科主题 | Immunology |
| 类目[WOS] | Immunology |
| 关键词[WOS] | NF-KAPPA-B ; TRANSCRIPTION FACTOR SPI-1/PU.1 ; IL-1-BETA GENE-EXPRESSION ; NECROSIS-FACTOR-ALPHA ; RECEPTOR ANTAGONIST ; IN-VIVO ; SEPSIS SYNDROME ; CONTACT HYPERSENSITIVITY ; RHEUMATOID-ARTHRITIS ; BETA-A |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000259220700001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1486]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, LM,Fei, ZL,Ren, JK,et al. Functional imaging of interleukin 1 beta expression in inflammatory process using bioluminescence imaging in transgenic mice[J]. BMC IMMUNOLOGY,2008,9(1):49-49. |
| APA | Li, LM.,Fei, ZL.,Ren, JK.,Sun, RL.,Liu, ZH.,...&Fei, J.(2008).Functional imaging of interleukin 1 beta expression in inflammatory process using bioluminescence imaging in transgenic mice.BMC IMMUNOLOGY,9(1),49-49. |
| MLA | Li, LM,et al."Functional imaging of interleukin 1 beta expression in inflammatory process using bioluminescence imaging in transgenic mice".BMC IMMUNOLOGY 9.1(2008):49-49. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。