Differential expression of neutrophilic granule proteins between Th1 and Th2 cells
文献类型：期刊论文
| 作者 | Xie, D; Liu, ZD; Li, ZH; Ji, YY; Chen, JW; Sun, B |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2007 |
| 卷号 | 39期号:1页码:67-72 |
| 关键词 | neutrophilic granule protein cystatin Th2 cell |
| 通讯作者 | Sun, B (reprint author), Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,bsun@sibs.ac.cn |
| 英文摘要 | T helper cell type 1 (Th1) and 2 (Th2) play central roles in immune regulation. To identify the novel genes differentially expressed between Th1 and Th2 cells, CD4+ T cells were isolated from DO11.10 transgenic mice and induced under Th1 or Th2 conditions. Microarray showed differential expression of neutrophilic granule proteins (NGP) between Th1 and Th2 cells. NGP was first identified as a myeloid-specific granule protein with homology to the cystatin superfamily. Here we confirmed greater expression of NGP in Th2 cells by reverse transcription-polymerase chain reaction and real-time polymerase chain reaction analysis. We also showed that the expression of NGP mRNA had a peak expression after 5 d culture under Th2- but not Th1-biasing conditions. Antibody against NGP was prepared, and in concert with the results of mRNA analysis, the level of NGP protein in Th2 cells detected by Western blot analysis was also higher than that in Th1 cells. GFP-NGP fusion proteins overexpressed in HeLa cells were localized to the cytoplasm. These results suggest NGP is a novel marker distinguishing Th2 from Th1 cells and maybe a novel cytokine secreted by Th2 cells. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | CYSTEINE PROTEASE INHIBITOR ; IMMUNE-RESPONSES ; CYSTATIN-SA ; PROLIFERATION ; MODULATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000243881000009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1510]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xie, D,Liu, ZD,Li, ZH,et al. Differential expression of neutrophilic granule proteins between Th1 and Th2 cells[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2007,39(1):67-72. |
| APA | Xie, D,Liu, ZD,Li, ZH,Ji, YY,Chen, JW,&Sun, B.(2007).Differential expression of neutrophilic granule proteins between Th1 and Th2 cells.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,39(1),67-72. |
| MLA | Xie, D,et al."Differential expression of neutrophilic granule proteins between Th1 and Th2 cells".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 39.1(2007):67-72. |
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