PKC inhibition is involved in trichosanthin-induced apoptosis in human chronic myeloid leukemia cell line K562
文献类型：期刊论文
| 作者 | Li, J; Xia, XC; Nie, HL; Smith, MA; Zhu, XW |
| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
 |
| 出版日期 | 2007 |
| 卷号 | 1770期号:1页码:63-70 |
| 关键词 | trichosanthin apoptosis calcium PKC caspase-3 |
| 通讯作者 | Nie, HL (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,hlnie@sibs.ac.cn ; Xiongwei.Zhu@case.edu |
| 英文摘要 | Trichosanthin (TCS), a type I ribosome-inactivating protein, induces cell death in various cell types including several turner cell lines. However, the mechanism remains largely uncharacterized. In this study, we investigated the possible mechanism underlying its cytotoxicity by using human chronic myeloid leukemia cell line K562. We found that TCS induced apoptosis in K562 cells in a time- and concentration-dependent manner and can be blocked by caspase-3 inhibitors. Interestingly, TCS treatment induced a transient elevation in intracellular calcium concentration and a slow increase in reactive oxygen species production, while calcium chelators and antioxidants had no obvious effect on TCS-induced apoptosis, suggesting that calcium changes and reactive oxygen species may not be involved in TCS-mediated apoptosis in K562 cells. Instead we found that TCS partly inhibited PKC activity. Indeed, the PKC activator, PMA, inhibited while the PKC inhibitor, calphostin c, enhanced TCS-induced apoptosis. These PKC modulators had similar effects on TCS-induced cleavage of caspase-3, and caspase-3 inhibitors prevented calphostin c-enhanced apoptosis induced by TCS. In summary, we conclude that TCS induces apoptosis in K562 cells partly via PKC inhibition and caspase-3 activation. (c) 2006 Elsevier B.V All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | PROTEIN-KINASE-C ; IONIZING-RADIATION ; MEDIATED APOPTOSIS ; PATHWAY ; ACTIVATION ; ALPHA ; ANTICANCER ; INDUCTION ; RIBOSOME ; SURVIVAL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000243631600009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1511]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, J,Xia, XC,Nie, HL,et al. PKC inhibition is involved in trichosanthin-induced apoptosis in human chronic myeloid leukemia cell line K562[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2007,1770(1):63-70. |
| APA | Li, J,Xia, XC,Nie, HL,Smith, MA,&Zhu, XW.(2007).PKC inhibition is involved in trichosanthin-induced apoptosis in human chronic myeloid leukemia cell line K562.BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,1770(1),63-70. |
| MLA | Li, J,et al."PKC inhibition is involved in trichosanthin-induced apoptosis in human chronic myeloid leukemia cell line K562".BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1770.1(2007):63-70. |
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