Regulation of acetylcholinesterase expression by calcium signaling during calcium ionophore A23187-and thapsigargin-induced apoptosis
文献类型：期刊论文
| 作者 | Zhu, H; Gao, W; Jiang, H; Jin, QH; Shi, YF; Tsim, KWK; Zhang, XJ |
| 刊名 | INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
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| 出版日期 | 2007 |
| 卷号 | 39期号:1页码:93-108 |
| 关键词 | acetylcholinesterase apoptosis Ca2+ mRNA stability transcriptional activity |
| 通讯作者 | Zhang, XJ (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,Lab Mol Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,xjzhang@sibs.ac.cn |
| 英文摘要 | We have recently reported that acetylcholinesterase expression was induced during apoptosis in various cell types. In the current study we provide evidence to suggest that the induction of acetylcholinesterase expression during apoptosis is regulated by the mobilization of intracellular Ca2+. During apoptosis, treatment of HeLa and MDA-MB-435s cells with the calcium ionophore A23187 resulted in a significant increase in acetylcholinesterase mRNA and protein levels. Chelation of intracellular Ca2+ by BAPTA-AM (1,2-bis-(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester), an intracellular Ca2+ chelator, inhibited acetyleholinesterase expression. A23187 also enhanced the stability of acetylcholinesterase mRNA and increased the activity of acetylcholinesterase promoter, effects that were blocked by BAPTA-AM. Perturbations of cellular Ca2+ homeostasis by thapsigargin resulted in the increase of acetylcholinesterase expression as well as acetylcholinesterase promoter activity during thapsigargin induced apoptosis in HeLa and MDA-MB-435s cells, effects that were also inhibited by BAPTA-AM. We further demonstrated that the transactivation of the human acetylcholinesterase promoter by A23187 and thapsigargin was partially mediated by a CCAAT motif within the -1270 to -1248 fragment of the human acetylcholinesterase promoter. This motif was able to bind to CCAAT binding factor (CBF/NF-Y). These results strongly suggest that cytosolic Ca2+ plays a key role in acetylcholinesterase regulation during apoptosis induced by A23187 and thapsigargin. (c) 2006 Elsevier Ltd. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | MESSENGER-RNA STABILITY ; AMYLOID-BETA-PEPTIDE ; INTRACELLULAR CALCIUM ; ENDOPLASMIC-RETICULUM ; TRANSCRIPTIONAL REGULATION ; MYOGENIC DIFFERENTIATION ; NEUROMUSCULAR-JUNCTIONS ; INHIBITOR THAPSIGARGIN ; NEUROBLASTOMA-CELLS ; HIPPOCAMPAL-NEURONS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000241963000009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1515]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhu, H,Gao, W,Jiang, H,et al. Regulation of acetylcholinesterase expression by calcium signaling during calcium ionophore A23187-and thapsigargin-induced apoptosis[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2007,39(1):93-108. |
| APA | Zhu, H.,Gao, W.,Jiang, H.,Jin, QH.,Shi, YF.,...&Zhang, XJ.(2007).Regulation of acetylcholinesterase expression by calcium signaling during calcium ionophore A23187-and thapsigargin-induced apoptosis.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,39(1),93-108. |
| MLA | Zhu, H,et al."Regulation of acetylcholinesterase expression by calcium signaling during calcium ionophore A23187-and thapsigargin-induced apoptosis".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 39.1(2007):93-108. |
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