A present-day aminoacyl-tRNA synthetase with ancestral editing properties
文献类型：期刊论文
| 作者 | Zhu, B; Zhao, MW; Eriani, G; Wang, ED |
| 刊名 | RNA-A PUBLICATION OF THE RNA SOCIETY
 |
| 出版日期 | 2007 |
| 卷号 | 13期号:1页码:15-21 |
| 关键词 | aminoacyl-tRNA synthetases evolution proofreading |
| 通讯作者 | Wang, ED (reprint author), 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,edwang@sibs.ac.cn |
| 英文摘要 | Leucyl-, isoleucyl-, and valyl-tRNA synthetases form a subgroup of related aminoacyl-tRNA synthetases that attach similar amino acids to their cognate tRNAs. To prevent amino acid misincorporation during translation, these enzymes also hydrolyze mischarged tRNAs through a post-transfer editing mechanism. Here we show that LeuRS from the deep-branching bacterium Aquifex aeolicus edits the complete set of aminoacylated tRNAs generated by the three enzymes: Ile- tRNA(Ile), Val-tRNA(Ile), Val-tRNA(Val), Thr-tRNA(Val), and Ile-tRNA(Leu). This unusual enlarged editing property was studied in a model of a primitive editing system containing a composite minihelix carrying the triple leucine, isoleucine, and valine identity mimicking the primitive tRNA precursor. We found that the freestanding LeuRS editing domain can edit this precursor in contrast to IleRS and ValRS editing domains. These results suggest that A. aeolicus LeuRS carries editing properties that seem more primitive than those of IleRS and ValRS. They suggest that the A. aeolicus editing domain has preserved the ambiguous editing property from the ancestral common editing domain or, alternatively, that this plasticity results from a specific metabolic adaptation. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | ESCHERICHIA-COLI ; AQUIFEX-AEOLICUS ; CRYSTAL-STRUCTURE ; CELL VIABILITY ; GENETIC-CODE ; YBAK PROTEIN ; DOMAIN ; DISCRIMINATION ; CYS-TRNA(PRO) ; ISOLEUCINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000243206800003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1516]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhu, B,Zhao, MW,Eriani, G,et al. A present-day aminoacyl-tRNA synthetase with ancestral editing properties[J]. RNA-A PUBLICATION OF THE RNA SOCIETY,2007,13(1):15-21. |
| APA | Zhu, B,Zhao, MW,Eriani, G,&Wang, ED.(2007).A present-day aminoacyl-tRNA synthetase with ancestral editing properties.RNA-A PUBLICATION OF THE RNA SOCIETY,13(1),15-21. |
| MLA | Zhu, B,et al."A present-day aminoacyl-tRNA synthetase with ancestral editing properties".RNA-A PUBLICATION OF THE RNA SOCIETY 13.1(2007):15-21. |
入库方式： OAI收割
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