Cell polarity protein Par3 complexes with DNA-PK via Ku70 and regulates DNA double-strand break repair
文献类型：期刊论文
| 作者 | Fang, LH; Wang, YG; Du, D; Yang, G; Kwok, TT; Kong, SK; Chen, B; Chen, DJ; Chen, ZJ |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2007 |
| 卷号 | 17期号:2页码:100-116 |
| 关键词 | cell polarity DSB repair DNA-PK Ku70/Ku80/Par3 |
| 通讯作者 | Chen, ZJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Proteom, Shanghai 200031, Peoples R China.,zjchen@sibs.ac.cn |
| 英文摘要 | The partitioning-defective 3 (Par3), a key component in the conserved Par3/Par6/aPKC complex, plays fundamental roles in cell polarity. Herein we report the identification of Ku70 and Ku80 as novel Par3 -interacting proteins through an in vitro binding assay followed by liquid chromatography-tandem mass spectrometry. Ku70/Ku80 proteins are two key regulatory subunits of the DNA-dependent protein kinase (DNA-PK), which plays an essential role in repairing double-strand DNA breaks (DSBs). We determined that the nuclear association of Par3 with Ku70/Ku80 was enhanced by gamma-irradiation (IR), a potent DSB inducer. Furthermore, DNA-PKcs, the catalytic subunit of DNA-PK, interacted with the Par3/Ku70/Ku80 complex in response to IR. Par3 over-expression or knockdown was capable of up- or down-regulating DNA-PK activity, respectively. Moreover, the Par3 knockdown cells were found to be defective in random plasmid integration, defective in DSB repair following IR, and radiosensitive, phenotypes similar to that of Ku70 knockdown cells. These findings identify Par3 as a novel component of the DNA-PK complex and implicate an unexpected link of cell polarity to DSB repair. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | KINASE CATALYTIC SUBUNIT ; TIGHT JUNCTION ; EPITHELIAL TIGHT ; MESENCHYMAL TRANSITION ; IONIZING-RADIATION ; NEURONAL POLARITY ; DAMAGE ; ATM ; AUTOPHOSPHORYLATION ; CONTACT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000245201400002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1526]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Fang, LH,Wang, YG,Du, D,et al. Cell polarity protein Par3 complexes with DNA-PK via Ku70 and regulates DNA double-strand break repair[J]. CELL RESEARCH,2007,17(2):100-116. |
| APA | Fang, LH.,Wang, YG.,Du, D.,Yang, G.,Kwok, TT.,...&Chen, ZJ.(2007).Cell polarity protein Par3 complexes with DNA-PK via Ku70 and regulates DNA double-strand break repair.CELL RESEARCH,17(2),100-116. |
| MLA | Fang, LH,et al."Cell polarity protein Par3 complexes with DNA-PK via Ku70 and regulates DNA double-strand break repair".CELL RESEARCH 17.2(2007):100-116. |
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