Developmental expression of Cyclin H and Cdk7 in zebrafish: the essential role of Cyclin H during early embryo development
文献类型：期刊论文
| 作者 | Liu, QY; Wu, ZL; Lv, WJ; Yan, YC; Li, YP |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2007 |
| 卷号 | 17期号:2页码:163-173 |
| 关键词 | zebrafish Cyclin H Cdk7 whole-mount in situ hybridization embryonic development midblastula transition |
| 通讯作者 | Li, YP (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,Lab Mol Cell Biol, Shanghai 200031, Peoples R China.,yipingli@sibs.ac.cn |
| 英文摘要 | Cyclin-dependent kinase 7 (Cdk7) is the catalytic subunit of the metazoan Cdk-activating kinase (CAK). Activation of Cdk7 requires its association with a regulatory subunit, Cyclin H. Although the Cdk7/Cyclin H complex has been implicated in the regulation of RNA polymerase in several species, the precise function of their orthologs in zebrafish has not been fully elucidated. In this study, we isolated from zebrafish blastula embryos two cDNAs encoding the orthologs of human Cyclin H and Cdk7, and examined the role of Cdk7/Cyclin H in zebrafish embryogenesis. Sequence analysis showed that the zebrafish Cyclin H and Cdk7 cDNAs encode proteins with 65% and 86% identity to the respective human orthologs. RT-PCR and whole-mount in situ hybridization analyses of their expression in unfertilized eggs, embryos and organs of adult fish suggested that Cyclin H and Cdk7 messages are maternally loaded. Our data also showed that their transcripts were detected throughout development. Distribution of Cyclin H transcripts was found to be ubiquitous during early stages of development and become restricted to the anterior neural tube, brain, eyes, procreate tissues, liver and heart by 5 days post-fertilization. Expression of a dominant-negative form of Cyclin H delayed the onset of zygotic transcription in the early embryo, resulting in apoptosis at 5 hours post-fertilization and leading to sever defects in tissues normally exhibiting high levels of Cyclin H expression. These results implicate Cyclin H in the regulation of the transcriptional machinery during midblastula, transition and suggest that it is an essential gene in early zebrafish larval development. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | ACTIVATING KINASE CAK ; ASSEMBLY FACTOR ; T-LOOP ; TRANSCRIPTION ; XENOPUS ; CELLS ; PHOSPHORYLATION ; TRANSITION ; APOPTOSIS ; MOUSE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000245201400006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1527]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, QY,Wu, ZL,Lv, WJ,et al. Developmental expression of Cyclin H and Cdk7 in zebrafish: the essential role of Cyclin H during early embryo development[J]. CELL RESEARCH,2007,17(2):163-173. |
| APA | Liu, QY,Wu, ZL,Lv, WJ,Yan, YC,&Li, YP.(2007).Developmental expression of Cyclin H and Cdk7 in zebrafish: the essential role of Cyclin H during early embryo development.CELL RESEARCH,17(2),163-173. |
| MLA | Liu, QY,et al."Developmental expression of Cyclin H and Cdk7 in zebrafish: the essential role of Cyclin H during early embryo development".CELL RESEARCH 17.2(2007):163-173. |
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