Preparation and characterization of polyclonal antibody against Xenopus PAPC
文献类型：期刊论文
| 作者 | Hu, RY; Peng, X; Chen, YL; Xin, L; Ding, XY |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2007 |
| 卷号 | 34期号:2页码:222-228 |
| 关键词 | Xenopus laevis Paraxial Protocadherin (PAPC) prokaryotic expression polyclonal antibody Western blotting immuno fluorescent staining |
| 通讯作者 | Ding, XY (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,Lab Mol & Cell Biol, Shanghai 200031, Peoples R China.,xyding@sunm.shcnc.ac.cn |
| 英文摘要 | Xenopus Paraxial Protocadherin (PAPC), which was initially identified in a screen for genes present in the Spemann organizer of Xenopus embryos, is required for gastrulation, somitogenesis and otic vesicle formation. In order to investigate its function in various developmental events, an antibody was prepared which could specifically recognize Xenopus PAPC. Glutathione S transferase (GST) expression system was used to express the fusion protein GST-PAPC. Rabbits were immunized with GST-PAPC fusion protein and the anti-PAPC polyclonal antibody was obtained. In dilution of 1:3 000 the antibody recognized a specific band in Western blotting analysis of FL-PAPC transfected HEK 293T cells lysates, which could be specifically blocked by pre-adsorption of prokaryotic expressed GST-PAPC fusion protein. Furthermore, by using immunofluorescence analysis the polyclonal antibody recognized membrane-bound PAPC in FL-PAPC transfected 293T cells and Xenopus animal cap cells. By Western blotting analysis, the endogenous 150 ku PAPC protein was detected in Xenopus embryos using the anti-PAPC antibody. Take together it could be concluded that a polyclonal antibody specifically against Xenopus PAPC was developed. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | PARAXIAL PROTOCADHERIN ; INTERACTS ; MESODERM ; DOMAIN |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000244317300016 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1529]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hu, RY,Peng, X,Chen, YL,et al. Preparation and characterization of polyclonal antibody against Xenopus PAPC[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2007,34(2):222-228. |
| APA | Hu, RY,Peng, X,Chen, YL,Xin, L,&Ding, XY.(2007).Preparation and characterization of polyclonal antibody against Xenopus PAPC.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,34(2),222-228. |
| MLA | Hu, RY,et al."Preparation and characterization of polyclonal antibody against Xenopus PAPC".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 34.2(2007):222-228. |
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