Study on beta-sheet structure formed by self-assembly of fibroin crystalline typical peptides using thioflavine T fluorescence probe
文献类型：期刊论文
| 作者 | Wang, JN; Lu, CD; Bai, L |
| 刊名 | ACTA CHIMICA SINICA
 |
| 出版日期 | 2007 |
| 卷号 | 65期号:2页码:111-115 |
| 关键词 | fibroin crystalline peptide beta-sheet ThT fluorescence probe urea |
| 通讯作者 | Wang, JN (reprint author), Suzhou Univ, Coll Mat Engn, Suzhou 215021, Peoples R China.,ywy_sd@sina.com.cn |
| 英文摘要 | To study the self-organization/self-assembly mechanism of silkworm fibroin based material, four kinds of structurally simple fibroin crystalline peptides, GXGAGAGXGA (X: A, S, Y, V), were designed and then incubated for 1 similar to 15 d to assemble by themselves. Their abilities to form, beta-sheet structure were studied by ThT fluorescence spectral analysis and urea denaturation methods. Results showed that: GA could form beta-sheet after incubation for 1 d; after the peptide was incubated for 12, 8, 14 and 13 d respectively (in the order of A, S, Y and V), the ratio of beta-sheet structure in the aggregation tended to be stable. Among the four peptides GS had the highest beta-sheet content, GA took the second place, GY and GV had the lowest content. The presence of urea in peptide solution had an obvious effect on the formation of beta-sheet. Urea (1 mol/L) could stop the forming of beta-sheet structure from GS, GY and GV in the process of their self-assembly. When the concentration of urea was over 2 mol/L, the formation of beta-sheet structure from GA could be affected, and the content of beta-sheet decreased proportionally with increase of urea concentration. |
| 学科主题 | Chemistry |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 关键词[WOS] | SILK FIBROIN ; BEHAVIOR ; AGGREGATION ; PROTEINS |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000244172300005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1530]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, JN,Lu, CD,Bai, L. Study on beta-sheet structure formed by self-assembly of fibroin crystalline typical peptides using thioflavine T fluorescence probe[J]. ACTA CHIMICA SINICA,2007,65(2):111-115. |
| APA | Wang, JN,Lu, CD,&Bai, L.(2007).Study on beta-sheet structure formed by self-assembly of fibroin crystalline typical peptides using thioflavine T fluorescence probe.ACTA CHIMICA SINICA,65(2),111-115. |
| MLA | Wang, JN,et al."Study on beta-sheet structure formed by self-assembly of fibroin crystalline typical peptides using thioflavine T fluorescence probe".ACTA CHIMICA SINICA 65.2(2007):111-115. |
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