中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Association of Dnmt3a and thymine DNA glycosylase links DNA methylation with base-excision repair

文献类型:期刊论文

作者Li, YQ; Zhou, PZ; Zheng, XD; Walsh, CP; Xu, GL
刊名NUCLEIC ACIDS RESEARCH
出版日期2007
卷号35期号:2页码:390-400
通讯作者Xu, GL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,glxu@sibs.ac.cn
英文摘要While methylcytosines serve as the fifth base encoding epigenetic information, they are also a dangerous endogenous mutagen due to their intrinsic instability. Methylcytosine undergoes spontaneous deamination, at a rate much higher than cytosine, to generate thymine. In mammals, two repair enzymes, thymine DNA glycosylase (TDG) and methyl-CpG binding domain 4 (MBD4), have evolved to counteract the mutagenic effect of methylcytosines. Both recognize G/T mismatches arising from methylcytosine deamination and initiate base-excision repair that corrects them to G/C pairs. However, the mechanism by which the methylation status of the repaired cytosines is restored has remained unknown. We show here that the DNA methyltransferase Dnmt3a interacts with TDG. Both the PWWP domain and the catalytic domain of Dnmt3a are able to mediate the interaction with TDG at its N-terminus. The interaction affects the enzymatic activity of both proteins: Dnmt3a positively regulates the glycosylase activity of TDG, while TDG inhibits the methylation activity of Dnmt3a in vitro. These data suggest a mechanistic link between DNA repair and remethylation at sites affected by methylcytosine deamination.
学科主题Biochemistry & Molecular Biology
类目[WOS]Biochemistry & Molecular Biology
关键词[WOS]METHYLTRANSFERASE DNMT3B ; TRANSITION MUTATIONS ; CYTOSINE METHYLATION ; CPG DINUCLEOTIDE ; PWWP DOMAIN ; SITES ; DEAMINATION ; PROTEIN ; GENE ; TRANSCRIPTION
收录类别SCI
语种英语
WOS记录号WOS:000243993600010
版本出版稿
源URL[http://202.127.25.143/handle/331003/1535]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
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GB/T 7714
Li, YQ,Zhou, PZ,Zheng, XD,et al. Association of Dnmt3a and thymine DNA glycosylase links DNA methylation with base-excision repair[J]. NUCLEIC ACIDS RESEARCH,2007,35(2):390-400.
APA Li, YQ,Zhou, PZ,Zheng, XD,Walsh, CP,&Xu, GL.(2007).Association of Dnmt3a and thymine DNA glycosylase links DNA methylation with base-excision repair.NUCLEIC ACIDS RESEARCH,35(2),390-400.
MLA Li, YQ,et al."Association of Dnmt3a and thymine DNA glycosylase links DNA methylation with base-excision repair".NUCLEIC ACIDS RESEARCH 35.2(2007):390-400.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

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