Intein-mediated expression is an effective approach in the study of beta-defensins
文献类型：期刊论文
| 作者 | Diao, H; Guo, CY; Lin, DH; Zhang, YL |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2007 |
| 卷号 | 357期号:4页码:840-846 |
| 关键词 | intein beta-defensin defensin fusion-expression host defense innate immunity antimicrobial activity |
| 通讯作者 | Zhang, YL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Key Lab Mol Androl, State Key Lab Mol Biol,Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,ylzhang@sibs.ac.cn |
| 英文摘要 | Mammalian beta-defensins are an important family of host defense peptides with diverse functions. Surprisingly most of the mammalian beta-defensin genes are revealed preferentially expressed in the male organs. There is a pressing need to understand how the ample defensin repertoires work in both host defense and fertility with an aim to overcome antibiotic resistance of pathogens and reproductive problems. The biggest obstacle is the production of beta-defensin peptides as P-defensins are small, antimicrobial and multi-disultide molecules. In this study, the well documented HBD2, function-unknown RBD1 and function-partly-known rBin1b are successfully expressed and assayed. This approach overcomes the difficulties in beta-defensin production and provides a convenient and economical peptide-production platform to elucidate the antimicrobial activities and clinical prospects of P-defensins. In the strategy of recombinant expression, this approach may be the best to develop the "natural" peptide pools for both host defense and fertility in a cost-effective manner. (c) 2007 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ESCHERICHIA-COLI ; HUMAN BETA-DEFENSIN-2 ; CANDIDA-ALBICANS ; ANTIMICROBIAL PEPTIDES ; PROTEIN-PURIFICATION ; RECOMBINANT PROTEINS ; INNATE ; GENE ; ANTIBIOTICS ; CLEAVAGE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000246648400004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1548]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Diao, H,Guo, CY,Lin, DH,et al. Intein-mediated expression is an effective approach in the study of beta-defensins[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2007,357(4):840-846. |
| APA | Diao, H,Guo, CY,Lin, DH,&Zhang, YL.(2007).Intein-mediated expression is an effective approach in the study of beta-defensins.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,357(4),840-846. |
| MLA | Diao, H,et al."Intein-mediated expression is an effective approach in the study of beta-defensins".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 357.4(2007):840-846. |
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