Monoclonal antibody against non-dominant epitopes of HBV e Ag was raised by antigen-antibody co-immunization
文献类型：期刊论文
| 作者 | Yan, LC; Jing, CY; Huang, CF; Ji, YY; Yao, G; Cai, XF; Sun, B |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2007 |
| 卷号 | 129期号:4页码:620-627 |
| 关键词 | hepatitis B virus (HBV) HBeAg co-immunization epitope anti-HBeAg immunoassay ELISA |
| 通讯作者 | Sun, B (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Mol Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,bsun@sibs.ac.cn |
| 英文摘要 | Detection of hepatitis B e antigen (HBeAg) in the sera of individuals infected with hepatitis B virus (HBV) can indicate both a high infectivity of the disease and a poor prognosis of disease treatment. Most of monoclonal antibodies raised against HBV e proteins interact with immuno-dominant epitopes, such as HBeAg-beta. In order to raise antibodies against non-dominant epitopes of HBV e protein, in this study, mice were immunized with both recombinant HBeAg (rHBeAg) and an anti-HBeAg antibody (EWB) recognizing a dominant antigenic epitope of HBeAg (HBeAg-beta epitope). With this strategy, we successfully selected two monoclonal antibodies, S-29-3 and S-72-3. Both S-29-3 and S-72-3 bind to recombinant HBeAg with a high affinity. The epitope mapping assay determined that the S-73-2 recognizes the N-terminal of HBeAg (1-118 aa) and the S-29-3 recognizes the C-terminal of HBeAg (91-149 aa). Further experiment showed that these two antibodies could be formed a pair-Abs that is used in detecting native HBeAg from the sera of HBV patients. The conclusion is that the developed method is useful to raise mAb against non-dominant epitopes in given Ag. (c) 2007 Elsevier B.V. All rights reserved. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | HEPATITIS-B-VIRUS ; IMMUNOCHEMICAL STRUCTURE ; ESCHERICHIA-COLI ; SERUM ; IDENTIFICATION ; DETERMINANTS ; IMMUNOASSAY ; RECOGNIZE ; MARKERS ; HBEAG |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000246572200005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1550]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yan, LC,Jing, CY,Huang, CF,et al. Monoclonal antibody against non-dominant epitopes of HBV e Ag was raised by antigen-antibody co-immunization[J]. JOURNAL OF BIOTECHNOLOGY,2007,129(4):620-627. |
| APA | Yan, LC.,Jing, CY.,Huang, CF.,Ji, YY.,Yao, G.,...&Sun, B.(2007).Monoclonal antibody against non-dominant epitopes of HBV e Ag was raised by antigen-antibody co-immunization.JOURNAL OF BIOTECHNOLOGY,129(4),620-627. |
| MLA | Yan, LC,et al."Monoclonal antibody against non-dominant epitopes of HBV e Ag was raised by antigen-antibody co-immunization".JOURNAL OF BIOTECHNOLOGY 129.4(2007):620-627. |
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