C/EBP alpha p30 plays transcriptional regulatory roles distinct from C/EBP alpha p42
文献类型：期刊论文
| 作者 | Wang, CX; Chen, XT; Wang, YP; Gong, JL; Hu, GX |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2007 |
| 卷号 | 17期号:4页码:374-383 |
| 关键词 | C/EBP alpha expression pattern p84N5 MPP11 SMYD2 |
| 通讯作者 | Hu, GX (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,hgxgene@sunm.shcnc.ac.cn |
| 英文摘要 | CCAAT/enhancer binding protein alpha (C/EBP alpha) is a transcriptional regulatory factor that inhibits cell proliferation, and alternative translational initiation produces two polypeptides, C/EBP alpha p30 and C/EBP alpha p42. By expression profiling, it was revealed that C/EBP alpha p30 specifically inhibited a unique set of genes, including MPP 11, p84N5 and SMYD2, which were not affected by C/EBP alpha p42 in both QSG-7701 hepatocyte cell line and QGY-7703 hepatoma cells. Semiquantitative RT-PCR analysis independently confirmed these results. Chromatin immunoprecipitation assay showed that C/EBP alpha p30 bound to the promoters of these genes more strongly than C/EBP alpha p42. In clinical hepatoma samples in which C/EBP alpha was downregulated, all three genes specifically inhibited by C/EBP alpha p30 were upregulated. However, repression of MPP 11, p84N5 and SMYD2 genes might not be directly involved in C/EBP alpha p30-mediated growth inhibition. Our data suggest that C/EBP alpha p30 regulates a unique set of target genes and is more than a dominant-negative regulator of C/EBP alpha p42. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | BINDING-PROTEIN-ALPHA ; LEUKEMIA-ASSOCIATED ANTIGENS ; ACUTE MYELOID LEUKEMIAS ; C/EBP-ALPHA ; MESSENGER-RNA ; CELL-PROLIFERATION ; ATTENUATOR DOMAIN ; DOWN-REGULATION ; NEWBORN MICE ; DNA-BINDING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000246609400022 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1554]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, CX,Chen, XT,Wang, YP,et al. C/EBP alpha p30 plays transcriptional regulatory roles distinct from C/EBP alpha p42[J]. CELL RESEARCH,2007,17(4):374-383. |
| APA | Wang, CX,Chen, XT,Wang, YP,Gong, JL,&Hu, GX.(2007).C/EBP alpha p30 plays transcriptional regulatory roles distinct from C/EBP alpha p42.CELL RESEARCH,17(4),374-383. |
| MLA | Wang, CX,et al."C/EBP alpha p30 plays transcriptional regulatory roles distinct from C/EBP alpha p42".CELL RESEARCH 17.4(2007):374-383. |
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