Conditional RNA interference achieved by Oct-1 POU/rtTA fusion protein activator and a modified TRE-mouse U6 promoter
文献类型：期刊论文
| 作者 | Fei, ZL; Chen, Z; Wang, ZG; Fei, J |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2007 |
| 卷号 | 354期号:4页码:906-912 |
| 关键词 | RNA interference Oct-1 tetracycline conditional inducible gene expression |
| 通讯作者 | Fei, J (reprint author), Chinese Acad Sci, SIBS, Model Organism Res Ctr,Lab Mol Cell Biol, Inst Biochem & Cell Biol, 7 Bldg,319 Yueyang Rd, Shanghai 200031, Peoples R China.,jfei@sibs.ac.cn |
| 英文摘要 | RNA interference (RNAi) is a powerful technique and is widely used to down-regulate expression of specific genes in cultured cells and in vivo. In this paper, we report our development of a new tetracycline-inducible RNAi expression using a modified TRE-mouse U6 promoter in which the distal sequence element (DSE) was replaced by the tetracycline-responsive element (TRE). The modified TRE-mouse U6 promoter can be activated by a Tet-on version tetracycline-regulated artificial activator rTetOct which was constructed by fusing the rtTA DNA binding domain with the Oct-1 POU activation domain. This rTetOct/TRE-U6 system was successfully applied to conditionally and reversibly down-regulate the expression of endogenous p53 gene in MCF7 cells, and the expression of P-defensin gene (mBin1b) either transiently expressed in COS7 cells or stably expressed in CHO cells. (c) 2007 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | MAMMALIAN-CELLS ; GENE-EXPRESSION ; SYSTEM ; KNOCKDOWN ; TRANSCRIPTION ; DOMAIN ; SNRNA ; BASAL ; RATS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000244411800011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1557]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Fei, ZL,Chen, Z,Wang, ZG,et al. Conditional RNA interference achieved by Oct-1 POU/rtTA fusion protein activator and a modified TRE-mouse U6 promoter[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2007,354(4):906-912. |
| APA | Fei, ZL,Chen, Z,Wang, ZG,&Fei, J.(2007).Conditional RNA interference achieved by Oct-1 POU/rtTA fusion protein activator and a modified TRE-mouse U6 promoter.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,354(4),906-912. |
| MLA | Fei, ZL,et al."Conditional RNA interference achieved by Oct-1 POU/rtTA fusion protein activator and a modified TRE-mouse U6 promoter".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 354.4(2007):906-912. |
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