Cultivation of recombinant Escherichia coli for secretory production of human epidermal growth factor under control of P-L promoter
文献类型：期刊论文
| 作者 | Zhang, HY; Li, ZM; Qian, Y; Zhang, QA; Du, P; Gan, RB; Ye, Q |
| 刊名 | ENZYME AND MICROBIAL TECHNOLOGY
 |
| 出版日期 | 2007 |
| 卷号 | 40期号:4页码:708-715 |
| 关键词 | Escherichia coli secretion human epidermal growth factor P-L promoter |
| 通讯作者 | Ye, Q (reprint author), E China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China.,qye@ecust.edu.cn |
| 英文摘要 | Human epidermal growth factor (hEGF) was expressed in Escherichia coli BL21 under the control of PL promoter and was secreted by using the alkaline phosphatase signal sequence. In batch cultivations, temperature was shifted up from 30 degrees C to one spanning 38-42 degrees C, and delay of the post-induction cell growth was observed, which resulted from expression of the cloned gene. The overall hEGF expression was enhanced with the increase in the induction temperature, while the ratio of extracellular to total hEGF showed an opposite trend. The highest ratio of extracellular hEGF was achieved when induced at 38 degrees C; about 45% of the total hEGF produced was secreted into the medium. Therefore, an induction temperature of 38 degrees C was adopted. In fed-batch cultivations, the pre-induction nutrition condition had a great influence on the initial hEGF productivity after the temperature up-shift, and supply of glucose at a constant specific rate of 0.196 g g(-1) h(-1) improved hEGF production and secretion. By using a rich medium, a final cell density of 26.6 g DCW l(-1) and extracellular hEGF production of 380 mg l(-1) were obtained. (c) 2006 Elsevier Inc. All rights reserved. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | GENE-EXPRESSION ; CONTINUOUS-CULTURE ; TEMPERATURE ; PROTEINS ; SYSTEM ; LEVEL ; FERMENTATION ; INHIBITION ; VECTOR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000245015600030 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1558]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, HY,Li, ZM,Qian, Y,et al. Cultivation of recombinant Escherichia coli for secretory production of human epidermal growth factor under control of P-L promoter[J]. ENZYME AND MICROBIAL TECHNOLOGY,2007,40(4):708-715. |
| APA | Zhang, HY.,Li, ZM.,Qian, Y.,Zhang, QA.,Du, P.,...&Ye, Q.(2007).Cultivation of recombinant Escherichia coli for secretory production of human epidermal growth factor under control of P-L promoter.ENZYME AND MICROBIAL TECHNOLOGY,40(4),708-715. |
| MLA | Zhang, HY,et al."Cultivation of recombinant Escherichia coli for secretory production of human epidermal growth factor under control of P-L promoter".ENZYME AND MICROBIAL TECHNOLOGY 40.4(2007):708-715. |
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