Intermediates in the refolding of urea-denatured dimeric arginine kinase from Stichopus japonicus
文献类型：期刊论文
| 作者 | Qin, G; Jianwei, Z; Taotao, L; Xicheng, W |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2007 |
| 卷号 | 41期号:5页码:521-528 |
| 关键词 | arginine kinase refolding equilibrium intermediate kinetic intermediate |
| 通讯作者 | Qin, G (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,qguo@sibs.ac.cn |
| 英文摘要 | The refolding of urea-denatured dimeric AK was investigated by both equilibrium and kinetic measurements. Both studies indicated that the refolding of dimeric AK is a multiphasic process. The equilibrium studies, monitored by enzyme activity, intrinsic protein fluorescence, circular dichroism (CD), 1-anilinonaphtalene-8-sulfonate (ANS) binding, size-exclusion chromatography and glutaraldehyde cross-linking showed that there were at least two intermediates involved in this process: I-1 (existing in 1.8-1.4M urea) and I-2 (existing in 0.8-0.4M urea). I-1 was a monomeric intermediate and possessed characteristic similar to the globular folding intermediates described in the literature. 12 was an active native-like intermediate. The kinetic studies suggested that the refolding of AK possessed a burst phase, fast phase and slow phase, which involved at least the burst phase intermediates (I-B). Comparison of the properties of these intermediates suggested that In in the kinetic process corresponded to I-1 in the equilibrium process. Based on these results, a scheme for refolding of urea-denatured AK was proposed. (C) 2007 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | MUSCLE CREATINE-KINASE ; MOLTEN GLOBULE STATE ; GUANIDINE-HYDROCHLORIDE ; EQUILIBRIUM ; PURIFICATION ; TEMPERATURE ; MECHANISM ; GDNHCL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000251068800005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1561]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Qin, G,Jianwei, Z,Taotao, L,et al. Intermediates in the refolding of urea-denatured dimeric arginine kinase from Stichopus japonicus[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2007,41(5):521-528. |
| APA | Qin, G,Jianwei, Z,Taotao, L,&Xicheng, W.(2007).Intermediates in the refolding of urea-denatured dimeric arginine kinase from Stichopus japonicus.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,41(5),521-528. |
| MLA | Qin, G,et al."Intermediates in the refolding of urea-denatured dimeric arginine kinase from Stichopus japonicus".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 41.5(2007):521-528. |
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