Production of human epidermal growth factor in fed-batch culture of acetate-tolerant Escherichia coli
文献类型：期刊论文
| 作者 | Han, Y; Li, ZM; Du, P; Gan, RB; Ye, Q |
| 刊名 | CHINESE JOURNAL OF CHEMICAL ENGINEERING
 |
| 出版日期 | 2007 |
| 卷号 | 15期号:5页码:760-764 |
| 关键词 | human epidermal growth factor acetate-tolerance Escherichia coli pH |
| 通讯作者 | Ye, Q (reprint author), E China Univ Chem Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China.,qye@ecust.edu.cn |
| 英文摘要 | An acetate-tolerant mutant of Escherichia coli DH5 alpha, DA19, was used for secretory production of human epidermal growth factor (hEGF) whose expression was under the control of phoA promoter. The recombinant cells were cultured in a chemically defined medium, and glucose was added at different specific provision rates during the growth and expression phases. It was found that pH had a significant effect on the extracellular hEGF production. The extracellular hEGF concentration was 75.5mg center dot L-1, 5.2-fold of the level reached at pH 7.0, even though more acetate was produced. Nitrogen source was limited in the later growth phase. Supplementation of ammonium promoted the consumption of phosphate and reduced the time to exhaust phosphate, but the extracellular hEGF production was similar to that without supplementation of ammonium. |
| 学科主题 | Engineering |
| 类目[WOS] | Engineering, Chemical |
| 关键词[WOS] | YK537 PAET-8 ; PH ; CULTIVATION ; SECRETION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000250819500025 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1562]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Han, Y,Li, ZM,Du, P,et al. Production of human epidermal growth factor in fed-batch culture of acetate-tolerant Escherichia coli[J]. CHINESE JOURNAL OF CHEMICAL ENGINEERING,2007,15(5):760-764. |
| APA | Han, Y,Li, ZM,Du, P,Gan, RB,&Ye, Q.(2007).Production of human epidermal growth factor in fed-batch culture of acetate-tolerant Escherichia coli.CHINESE JOURNAL OF CHEMICAL ENGINEERING,15(5),760-764. |
| MLA | Han, Y,et al."Production of human epidermal growth factor in fed-batch culture of acetate-tolerant Escherichia coli".CHINESE JOURNAL OF CHEMICAL ENGINEERING 15.5(2007):760-764. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。