Bcl-2 cleavages at two adjacent sites by different caspases promote cisplatin-induced apoptosis
文献类型：期刊论文
| 作者 | Zhu, JB; Yang, Y; Wu, JR |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2007 |
| 卷号 | 17期号:5页码:441-448 |
| 关键词 | Bcl-2 apoptosis cisplatin caspase-3 caspase-8 |
| 通讯作者 | Wu, JR (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Syst Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,wujr@sibs.ac.cn |
| 英文摘要 | The protein encoded by bcl-2 proto-oncogene plays an important role in the mitochondria-mediated apoptotic pathway. Although the general role of Bcl-2 is anti-apoptotic, previous work showed that Bcl-2 fragments cleaved by caspases could promote apoptotic process. We report herein that Bcl-2 protein was cleaved to produce two fragments of around 23 kDa in human hepatocarcinoma. BEL-7404 cells or in Bcl-2 overexpressing CHO cells induced by cisplatin. Treating cells with the general caspase inhibitor z-VAD-fmk blocked the induced cleavage of Bcl-2. Mutagenesis analyses showed that Bcl-2 was cleaved by caspases at two adjacent recognition sites in the loop domain (YEWD31 down arrow AGD(34)down arrow V), which could be inhibited by caspase-8 and -3 inhibitors, respectively. Overexpression of the carboxyl terminal 23 kDa fragments increased the sensitivity of CHO cells to cisplatin-induced apoptosis. These results indicate that Bcl-2 can be cleaved into two close fragments by different caspases during cisplatin-induced apoptosis, both of which contribute to the acceleration of apoptotic process. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | PROGRAMMED CELL-DEATH ; CYTOCHROME-C RELEASE ; DNA FRAGMENTATION ; INHIBITION ; PROTEASE ; INACTIVATION ; ASSOCIATION ; REGULATORS ; MEMBRANE ; PATHWAY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000247525700008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1568]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhu, JB,Yang, Y,Wu, JR. Bcl-2 cleavages at two adjacent sites by different caspases promote cisplatin-induced apoptosis[J]. CELL RESEARCH,2007,17(5):441-448. |
| APA | Zhu, JB,Yang, Y,&Wu, JR.(2007).Bcl-2 cleavages at two adjacent sites by different caspases promote cisplatin-induced apoptosis.CELL RESEARCH,17(5),441-448. |
| MLA | Zhu, JB,et al."Bcl-2 cleavages at two adjacent sites by different caspases promote cisplatin-induced apoptosis".CELL RESEARCH 17.5(2007):441-448. |
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