In vitro insulin refolding: Characterization of the intermediates and the putative folding pathway
文献类型：期刊论文
| 作者 | YueHua, T; Shuai, W; Yan, C; GenJun, X; YouMin, F |
| 刊名 | SCIENCE IN CHINA SERIES C-LIFE SCIENCES
 |
| 出版日期 | 2007 |
| 卷号 | 50期号:6页码:717-725 |
| 关键词 | insulin insulin refolding folding intermediate folding pathway disulfide |
| 通讯作者 | YouMin, F (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,fengym@sunm.shcnc.ac.cn |
| 英文摘要 | The in vitro refolding process of the double-chain insulin was studied based on the investigation of in vitro single-chain insulin refolding. Six major folding intermediates, named P1A, P2B, P3A, P4B, P5B, and P6B, were captured during the folding process. The refolding experiments indicate that all of these intermediates are on-pathway. Based on these intermediates and the formation of hypothetic transients, we propose a two-stage folding pathway of insulin. (1) At the early stage of the folding process, the reduced A chain and B chain individually formed the intermediates: two A chain intermediates (P1A and P3A), and four B chain intermediates (P2B, P4B, P5B, and P6B). (2) In the subsequent folding process, transient I was formed from P3A through thiol/disulfide exchange reaction; then, transients II and III, each containing two native disulfides, were formed through the recognition and interaction of transient I with P4B or P6B and the thiol group's oxidation reaction mainly using GSSG as oxidative reagent; finally, transients II and III, through thiol/mixture disulfide exchange reaction, formed the third native disulfide of insulin to complete the folding. |
| 学科主题 | Life Sciences & Biomedicine - Other Topics |
| 类目[WOS] | Biology |
| 关键词[WOS] | DISULFIDE-FORMING PATHWAY ; PHENYLALANYL CHAINS ; HUMAN PROINSULIN ; RIBONUCLEASE-A ; C-PEPTIDE ; B CHAINS ; REGENERATION ; RESYNTHESIS ; PRECURSOR ; PROTEINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000251020100001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1572]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | YueHua, T,Shuai, W,Yan, C,et al. In vitro insulin refolding: Characterization of the intermediates and the putative folding pathway[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2007,50(6):717-725. |
| APA | YueHua, T,Shuai, W,Yan, C,GenJun, X,&YouMin, F.(2007).In vitro insulin refolding: Characterization of the intermediates and the putative folding pathway.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,50(6),717-725. |
| MLA | YueHua, T,et al."In vitro insulin refolding: Characterization of the intermediates and the putative folding pathway".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 50.6(2007):717-725. |
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