Molecular and biochemical characterization of Ba-EGA, a cellulase secreted by Bacillus sp AC-1 from Ampullaria crosseans
文献类型：期刊论文
| 作者 | Zhang, S; Yin, QY; Li, YH; Ding, M; Xu, GJ; Zhao, FK |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2007 |
| 卷号 | 75期号:6页码:1327-1334 |
| 关键词 | gene cloning expression carbohydrate binding module non-covalent interactions |
| 通讯作者 | Zhao, FK (reprint author), Zheijiang Sci Tech Univ, Coll Life Sci, Hangzhou 310018, Peoples R China.,fkzhao@sibs.ac.cn |
| 英文摘要 | A novel gene (Ba-ega) of Bacillus sp. AC-1, encoding an endoglucanase (Ba-EGA), was cloned and expressed in Escherichia coli. Ba-ega, containing a 1,980-bp open reading frame (ORF), encoded a protein of 659 amino acids and had a molecular mass of 74.87 kDa. Ba-EGA was a modular enzyme composed of a family-9 glycosyl hydrolase catalytic module (CM9) and a family-3 carbohydrate-binding module (CBM3). To investigate the functions of the CBM3 and CM9, a number of truncated derivatives of Ba-EGA were constructed, and all were active. The catalytic module (rCM9) alone was less stable at high temperature than the recombinant Ba-EGA (rBa-EGA). The temperature stability for the complex of rCM9 and rCBM3 was still lower than rBa-EGA, but higher than rCM9 alone. These observations indicated the existence of a non-covalent interaction between CM9 and CBM3 that might strengthen the stability of CM9. However, this interaction is not strong enough to mimic the protective effect of the CBM in the wild-type enzyme. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | AMINO-ACID-SEQUENCE ; CLOSTRIDIUM-THERMOCELLUM ; MULTIDOMAIN ENDOGLUCANASE ; GLYCOSYL HYDROLASES ; PROTEIN HOMOLOGY ; SWISS-MODEL ; CLONING ; CELLULOSOME ; ENDO-BETA-1,4-GLUCANASE ; CELLULOLYTICUM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000247463100013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1575]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, S,Yin, QY,Li, YH,et al. Molecular and biochemical characterization of Ba-EGA, a cellulase secreted by Bacillus sp AC-1 from Ampullaria crosseans[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2007,75(6):1327-1334. |
| APA | Zhang, S,Yin, QY,Li, YH,Ding, M,Xu, GJ,&Zhao, FK.(2007).Molecular and biochemical characterization of Ba-EGA, a cellulase secreted by Bacillus sp AC-1 from Ampullaria crosseans.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,75(6),1327-1334. |
| MLA | Zhang, S,et al."Molecular and biochemical characterization of Ba-EGA, a cellulase secreted by Bacillus sp AC-1 from Ampullaria crosseans".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 75.6(2007):1327-1334. |
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