Two potent alpha 3/5 Conotoxins from piscivorous Conus achatinus
文献类型：期刊论文
| 作者 | Liu, L; Chew, G; Hawrot, E; Chi, CW; Wang, CG |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2007 |
| 卷号 | 39期号:6页码:438-444 |
| 关键词 | cone snail alpha 3/5 conotoxin disulfide bond nicotinic acetylcholine receptor |
| 通讯作者 | Wang, CG (reprint author), Tongji Univ, Inst Prot Res, Shanghai 200092, Peoples R China.,chunguangwang@mail.tongji.edu.cn |
| 英文摘要 | Every cone snail produces a mixture of different conotoxins and secretes them to immobilize their prey and predators. alpha 3/5 Conotoxins, isolated from fish-hunting cone snails, target muscle nicotinic acetylcholine receptors. The structure and function of alpha 3/5 conotoxin from the piscivorous Conus achatinus have not been studied. We synthesized two pentadecamer peptides, Ac1.1a and Ac 1.1 b, with appropriate disulfide bonding, based on cDNA sequences of alpha 3/5 conotoxins from C. achatinus. Ac 1.1 a and Ac 1.1 b differ by only one amino acid residue. They have similar potency on blocking recombinant mouse muscle acetylcholine receptor expressed in Xenopus laevis oocytes, with IC50 values of 36 nM and 26 nM, respectively. For Ac1.1 b, deletion of the first three N-terminal amino acids did not change its activity, indicating that the N-terminus is not involved in the interaction with its receptor. Furthermore, our experiments indicate that both toxins strongly prefer the alpha(1)-delta subunit interface instead of the alpha(1)-gamma binding site on the mouse muscle nicotinic acetylcholine receptor. These peptides provide additional tools for the study of the structure and function of nicotinic receptor. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | NICOTINIC ACETYLCHOLINE-RECEPTOR ; PEPTIDE TOXINS ; HOMOLOG ACHBP ; BINDING-SITE ; MI ; BUNGAROTOXIN ; GI ; SELECTIVITY ; GEOGRAPHUS ; EVOLUTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000247529600007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1576]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, L,Chew, G,Hawrot, E,et al. Two potent alpha 3/5 Conotoxins from piscivorous Conus achatinus[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2007,39(6):438-444. |
| APA | Liu, L,Chew, G,Hawrot, E,Chi, CW,&Wang, CG.(2007).Two potent alpha 3/5 Conotoxins from piscivorous Conus achatinus.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,39(6),438-444. |
| MLA | Liu, L,et al."Two potent alpha 3/5 Conotoxins from piscivorous Conus achatinus".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 39.6(2007):438-444. |
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