NDST-1 modulates BMPR and PTHrP signaling during endochondral bone formation in a gene knockout model
文献类型：期刊论文
| 作者 | Hu, ZH; Yu, MY; Hu, GX |
| 刊名 | BONE
 |
| 出版日期 | 2007 |
| 卷号 | 40期号:6页码:1462-1474 |
| 关键词 | BMP delay endochondral bone formation NDST-1 PTHrP |
| 通讯作者 | Hu, GX (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,hgxgene@sunm.shenc.ac.cn |
| 英文摘要 | GlcNAc N-deacetylase/N-sulfotransferase-1 (NDST-1), a member of the enzyme family catalyzing the first modification step in the biosynthesis of heparan sulfate (HS), was knocked out in mice to investigate its role in embryonic development. NDST-I null mice exhibited delayed endochondral bone formation including shortened calcified zones in limbs, delayed chondrocyte and osteogenetic differentiation, and increased chondrocyte proliferation. In situ HS binding assay revealed that the binding ability of bone morphogenetic protein (BMP) -2, -4, and -6 to endogenous HS was decreased in mutant phalanges, while that of fibroblast growth factor-1 (FGF-1) was not affected. Up-regulation of BMPR-IA, Phospho-Smad1 (P-Smad1) and parathyroid-horrnone related protein (PTHrP), but riot the Indian hedgehog, Gli1, GO, Patched, and FGFR-3, was observed. Furthermore, block of BMPR signaling with noggin rescued the delayed chondrocyte hypertrophic differentiation in NDST-1 (-/-) mice and recovered the expression of both P-Smad1 and PTHrP proteins. These results suggested that NDST-1-dependent heparan sulfate might negatively modulate BMP and its downstream PTHrP signaling, and thus affect endochondral bone development. (c) 2007 Elsevier Inc. All rights reserved. |
| 学科主题 | Endocrinology & Metabolism |
| 类目[WOS] | Endocrinology & Metabolism |
| 关键词[WOS] | HEPARAN-SULFATE PROTEOGLYCANS ; HORMONE-RELATED PEPTIDE ; N-DEACETYLASE/N-SULFOTRANSFERASE ; FIBROBLAST-GROWTH-FACTOR ; CELL-SURFACE ; OSTEOBLAST DIFFERENTIATION ; CHONDROCYTE PROLIFERATION ; CARTILAGE DIFFERENTIATION ; MORPHOGENETIC PROTEINS ; TARGETED DISRUPTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000247423600004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1577]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hu, ZH,Yu, MY,Hu, GX. NDST-1 modulates BMPR and PTHrP signaling during endochondral bone formation in a gene knockout model[J]. BONE,2007,40(6):1462-1474. |
| APA | Hu, ZH,Yu, MY,&Hu, GX.(2007).NDST-1 modulates BMPR and PTHrP signaling during endochondral bone formation in a gene knockout model.BONE,40(6),1462-1474. |
| MLA | Hu, ZH,et al."NDST-1 modulates BMPR and PTHrP signaling during endochondral bone formation in a gene knockout model".BONE 40.6(2007):1462-1474. |
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