Nudel modulates kinetochore association and function of cytoplasmic dynein in M phaseli
文献类型：期刊论文
| 作者 | Liang, Y; Yu, W; Li, Y; Yu, LH; Zhang, QG; Wang, FB; Yang, ZY; Du, J; Huang, QP; Yao, XB |
| 刊名 | MOLECULAR BIOLOGY OF THE CELL
 |
| 出版日期 | 2007 |
| 卷号 | 18期号:7页码:2656-2666 |
| 通讯作者 | Zhu, XL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China.,xlzhu@sibs.ac.cn |
| 英文摘要 | The microtubule-based motor cytoplasmic dynein/dynactin is a force generator at the kinetochore. It also transports proteins away from kinetochores to spindle poles. Regulation of such diverse functions, however, is poorly understood. We have previously shown that Nudel is critical for dynein-mediated protein transport, whereas mitosin, a kinetochore protein that binds Nudel, is involved in retention of kinetochore dynein/dynactin against microtubule-dependent stripping. Here we demonstrate that Nudel is required for robust localization of dynein/dynactin at the kinetochore. It localizes to kinetochores after nuclear envelope breakdown, depending mostly (similar to 78%) on mitosin and slightly on dynein/dynactin. Depletion of Nudel by RNA interference (RNAi) or overexpression of its mutant incapable of binding either Lisl or dynein heavy chain abolishes the kinetochore protein transport and mitotic progression. Similar to mitosin RNAi, Nudel RNAi also leads to increased stripping of kinetochore dynein/dynactin in the presence of microtubules. Taking together, our results suggest a dual role of kinetochore Nudel: it activates dynein-mediated protein transport and, when interacting with both mitosin and dynein, stabilizes kinetochore dynein/dynactin against microtubule-dependent stripping to facilitate the force generation function of the motor. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | SPINDLE CHECKPOINT ; MITOTIC SPINDLE ; CENP-F ; MITOSIN/CENP-F ; CHROMOSOME ALIGNMENT ; NEURONAL MIGRATION ; PROTEIN-TRANSPORT ; MAMMALIAN-CELLS ; DYNACTIN ; LIS1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000247604000026 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1582]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liang, Y,Yu, W,Li, Y,et al. Nudel modulates kinetochore association and function of cytoplasmic dynein in M phaseli[J]. MOLECULAR BIOLOGY OF THE CELL,2007,18(7):2656-2666. |
| APA | Liang, Y.,Yu, W.,Li, Y.,Yu, LH.,Zhang, QG.,...&Zhu, XL.(2007).Nudel modulates kinetochore association and function of cytoplasmic dynein in M phaseli.MOLECULAR BIOLOGY OF THE CELL,18(7),2656-2666. |
| MLA | Liang, Y,et al."Nudel modulates kinetochore association and function of cytoplasmic dynein in M phaseli".MOLECULAR BIOLOGY OF THE CELL 18.7(2007):2656-2666. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。