Kinetochore dynein generates a poleward pulling force to facilitate congression and full chromosome alignment
文献类型：期刊论文
| 作者 | Li, Y; Yu, W; Liang, Y; Zhu, XL |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2007 |
| 卷号 | 17期号:8页码:701-712 |
| 关键词 | kinetochore cytoplasmic dynein chromosome mitosis force |
| 通讯作者 | Zhu, XL (reprint author), Chinese Acad Sci, Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China.,xlzhu@sibs.ac.cn |
| 英文摘要 | For proper chromosome segregation, all kinetochores must achieve bipolar microtubule (MT) attachment and subsequently align at the spindle equator before anaphase onset. The MT minus end-directed motor dynein/dynactin binds kinetochores in prometaphase and has long been implicated in chromosome congression. Unfortunately, inactivation of dynein usually disturbs spindle organization, thus hampering evaluation of its kinetochore roles. Here we specifically eliminated kinetochore dynein/dynactin by RNAi-mediated depletion of ZW 10, a protein essential for kinetochore localization of the motor. Time-lapse microscopy indicated markedly-reduced congression efficiency, though congressing chromosomes displayed similar velocities as in control cells. Moreover, cells frequently failed to achieve full chromosome alignment, despite their normal spindles. Confocal microcopy revealed that the misaligned kinetochores were monooriented or unattached and mostly lying outside the spindle, suggesting a difficulty to capture MTs from the opposite pole. Kinetochores on monoastral spindles were dispersed farther away from the pole and exhibited only mild oscillation. Furthermore, inactivating dynein by other means generated similar phenotypes. Therefore, kinetochore dynein produces on monooriented kinetochores a poleward pulling force, which may contribute to efficient bipolar attachment by facilitating their proper microtubule captures to promote congression as well as full chromosome alignment. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | SMALL-MOLECULE INHIBITOR ; CYTOPLASMIC DYNEIN ; MITOTIC CHECKPOINT ; SPINDLE CHECKPOINT ; MAMMALIAN-CELLS ; CENP-E ; PROTEIN-TRANSPORT ; MITOSIS ; ZW10 ; DYNACTIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000249774500006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1588]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, Y,Yu, W,Liang, Y,et al. Kinetochore dynein generates a poleward pulling force to facilitate congression and full chromosome alignment[J]. CELL RESEARCH,2007,17(8):701-712. |
| APA | Li, Y,Yu, W,Liang, Y,&Zhu, XL.(2007).Kinetochore dynein generates a poleward pulling force to facilitate congression and full chromosome alignment.CELL RESEARCH,17(8),701-712. |
| MLA | Li, Y,et al."Kinetochore dynein generates a poleward pulling force to facilitate congression and full chromosome alignment".CELL RESEARCH 17.8(2007):701-712. |
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