Glut-4 is translocated to both caveolae and non-caveolar lipid rafts, but is partially internalized through caveolae in insulin-stimulated adipocytes
文献类型：期刊论文
| 作者 | Yuan, T; Hong, S; Yao, Y; Liao, K |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2007 |
| 卷号 | 17期号:9页码:772-782 |
| 关键词 | Glut-4 translocation Glut-4 internalization lipid rafts caveolae sucrose density gradient flotation |
| 通讯作者 | Liao, K (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,kliao@sibs.ac.cn |
| 英文摘要 | Caveolae and non-caveolar lipid rafts are two types of membrane lipid microdomains that play important roles in insulin-stimulated glucose uptake in adipocytes. In order to ascertain their specific functions in this process, caveolae were ablated by caveolin-1 RNA interference. In Cav-1 RNAi adipocytes, neither insulin-stimulated glucose uptake nor Glut-4 (glucose transporter 4) translocation to membrane lipid microdomains was affected by the ablation of caveolae. With a modified sucrose density gradient, caveolae and non-caveolar lipid rafts could be separated. In the wild-type 3T3-L1 adipocytes, Glut-4 was found to be translocated into both caveolae and non-caveolar lipid rafts. However, in Cav-1 RNAi adipocytes, Glut-4 was localized predominantly in non-caveolar lipid rafts. After the removal of insulin, caveolae-localized Glut-4 was internalized faster than non-caveolar lipid raft-associated Glut-4. The internalization of Glut-4 from plasma membrane was significantly decreased in Cav-1 RNAi adipocytes. These results suggest that insulin-stimulated Glut-4 translocation and glucose uptake are caveolae-independent events. Caveolae play a role in the internalization of Glut-4 from plasma membrane after the removal of insulin. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | EPIDERMAL-GROWTH-FACTOR ; 3T3-L1 ADIPOCYTES ; ADIPOSE-CELLS ; CAVEOLIN-1-DEFICIENT MICE ; MEMBRANE TRAFFICKING ; GLUCOSE-TRANSPORTER ; SIGNAL-TRANSDUCTION ; PLASMA-MEMBRANE ; RECEPTOR ; DIFFERENTIATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000250454800005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1594]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yuan, T,Hong, S,Yao, Y,et al. Glut-4 is translocated to both caveolae and non-caveolar lipid rafts, but is partially internalized through caveolae in insulin-stimulated adipocytes[J]. CELL RESEARCH,2007,17(9):772-782. |
| APA | Yuan, T,Hong, S,Yao, Y,&Liao, K.(2007).Glut-4 is translocated to both caveolae and non-caveolar lipid rafts, but is partially internalized through caveolae in insulin-stimulated adipocytes.CELL RESEARCH,17(9),772-782. |
| MLA | Yuan, T,et al."Glut-4 is translocated to both caveolae and non-caveolar lipid rafts, but is partially internalized through caveolae in insulin-stimulated adipocytes".CELL RESEARCH 17.9(2007):772-782. |
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