A novel method for assaying enzymatic activity of ribosome-inactivating protein using plasmid DNA as substrate
文献类型：期刊论文
| 作者 | Hong-Tao, W; Shao-Ling, Z; Wang-Yi, L |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2007 |
| 卷号 | 34期号:9页码:991-995 |
| 关键词 | ribosome-inactivating protein(RIP) double-stranded supercoiled DNA gel electrophoresis |
| 通讯作者 | Shao-Ling, Z (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,nnzsl@njau.edu.cn ; wyliu@sibs.ac.an |
| 英文摘要 | A novel method for assaying the enzymatic activity of ribosome-inactivating proteins (RIPs) has been developed. The principle of the method is based on that RIP can remove some adenine bases from double-stranded supercoiled DNA molecules, subsequently, the deadenylated DNA was cleaved into nicked and linear form. After treatment with acidic aniline, the deadenylated DNA was degraded into many small fragments, and run out of the gel. The enzymatic activities of two RIPs (trichosanthin and cinnamomin) were tested using this method, the limit of sensitivity is about 50 ng (trichosanthin) and 5 ng (reduced cinnamomin). It should be emphasized that the merit of this method is to avoid the preparation of ribosome. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | RNA N-GLYCOSIDASE ; EUKARYOTIC RIBOSOMES ; CINNAMOMUM-CAMPHORA ; SUPERCOILED DNA ; MECHANISM ; RICIN ; CHAIN |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000249601800013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1596]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hong-Tao, W,Shao-Ling, Z,Wang-Yi, L. A novel method for assaying enzymatic activity of ribosome-inactivating protein using plasmid DNA as substrate[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2007,34(9):991-995. |
| APA | Hong-Tao, W,Shao-Ling, Z,&Wang-Yi, L.(2007).A novel method for assaying enzymatic activity of ribosome-inactivating protein using plasmid DNA as substrate.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,34(9),991-995. |
| MLA | Hong-Tao, W,et al."A novel method for assaying enzymatic activity of ribosome-inactivating protein using plasmid DNA as substrate".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 34.9(2007):991-995. |
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