A proteomic-based approach for the characterization of some major structural proteins involved in host-parasite relationships from the silkworm parasite Nosema bombycis (Microsporidia)
文献类型：期刊论文
| 作者 | Wang, JY; Chambon, C; Lu, CD; Huang, KW; Vivares, CP; Texier, C |
| 刊名 | PROTEOMICS
 |
| 出版日期 | 2007 |
| 卷号 | 7期号:9页码:1461-1472 |
| 关键词 | 2-DE maps microsporiclia MS/MS novo sequencing Nosema bombycis polar tube proteins |
| 通讯作者 | Texier, C (reprint author), Univ Clermont Ferrand, CNRS, UMR 6023, LBP,Equipe Parasitol Mol & Cellulaire, F-63177 Aubiere, France.,catherine.texier@univ-bpclermont.fr |
| 英文摘要 | Nosema bombycis is the causative agent of the silkworm Bombyx mori pebrine disease which inflicts severe worldwide economical losses in sericulture. Little is known about host-parasite interactions at the molecular level for this spore-forming obligate intracellular parasite which belongs to the fungi-related Microsporidia phylum. Major microsporidian structural proteins from the spore wall (SW) and the polar tube (PT) are known to be involved in host invasion. We developed a proteomic-based approach to identify few N. bombycis proteins belonging to these cell structures. Protein extraction protocols were optimized and four N. bombycis spore protein extracts were compared by SDS-PAGE and 2-DE to establish complementary proteomic profiles. Three proteins were shown to be located at the parasite SW Moreover, 17 polyclonal antibodies were raised against major N. bombycis proteins from all extracts, and three spots were shown to correspond to polar tube proteins (PTPs) by immunofluorescent assay and transmission electron microscopy immunocytochernistry on cryosections. Specific patterns for each PTP were obtained by MALDI-TOF-MS and MS/MS. Peptide sequence tags were deduced by de novo sequencing using Peaks Online and DeNovoX, then evaluated by MASCOT and SEQUEST searches. Identification parameters were higher than false-positive hits, strengthening our strategy that could be enlarged to a nongenomic context. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology |
| 关键词[WOS] | POLAR TUBE PROTEIN ; ENCEPHALITOZOON-CUNICULI MICROSPORIDIA ; SPORE WALL PROTEIN ; GENOME SEQUENCE ; DEVELOPMENTAL EXPRESSION ; MASS-SPECTROMETRY ; FUNGAL ORIGIN ; GENES ; MORI ; IDENTIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000246635600010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1597]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, JY,Chambon, C,Lu, CD,et al. A proteomic-based approach for the characterization of some major structural proteins involved in host-parasite relationships from the silkworm parasite Nosema bombycis (Microsporidia)[J]. PROTEOMICS,2007,7(9):1461-1472. |
| APA | Wang, JY,Chambon, C,Lu, CD,Huang, KW,Vivares, CP,&Texier, C.(2007).A proteomic-based approach for the characterization of some major structural proteins involved in host-parasite relationships from the silkworm parasite Nosema bombycis (Microsporidia).PROTEOMICS,7(9),1461-1472. |
| MLA | Wang, JY,et al."A proteomic-based approach for the characterization of some major structural proteins involved in host-parasite relationships from the silkworm parasite Nosema bombycis (Microsporidia)".PROTEOMICS 7.9(2007):1461-1472. |
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