Induction of a high population of neural stem cells with anterior neuroectoderm characters from epiblast-like P19 embryonic carcinoma cells
文献类型：期刊论文
| 作者 | Xia, CH; Wang, C; Zhang, KJ; Qian, C; Jing, NH |
| 刊名 | DIFFERENTIATION
 |
| 出版日期 | 2007 |
| 卷号 | 75期号:10页码:912-927 |
| 关键词 | epiblast neural stem cell P19 cell anterior neuroectoderm neural induction N2B27 BMP and FGF signaling |
| 通讯作者 | Jing, NH (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci,Lab Mol Cell Biol, Inst Biochem & Cell Biol,Key Lab Stem Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,njing@sibs.ac.cn |
| 英文摘要 | The epiblast, derived from the inner cell mass (ICM), represents the final embryonic founder cell population of mouse embryo and can give rise to all germ layer lineages including the neuroectoderm. The generation of neural stem cells from epiblast-like cells is of great value for studying the mechanism of neural determination during gastrulation stages of embryonic development. Mouse embryonic carcinoma (EC) P19 cells are equivalent to the epiblast of early post-implantation blastocysts. In this study, we establish a feasible induction system that allows rapid and efficient derivation of a high percentage (similar to 95%) of neural stem cells from P19 EC cell in N2B27 serum-free medium. The induced neural stem cells bear anterior neuroectoderm characters, and can be efficiently caudalized by retinoic acid (RA). These neural stem cells have multilineage potential to differentiate into neurons, astrocytes, and oligodendrocytes. Mechanistic analysis indicates that inhibition of the bone morphogenetic protein (BMP) pathway may be the main reason for N2B27-neural induction, and that fibroblast growth factor (FGF) signaling is also involved in this process. This method will provide an in vitro system to dissect the molecular mechanisms involved in neural induction of early mouse embryos. |
| 学科主题 | Cell Biology; Developmental Biology |
| 类目[WOS] | Cell Biology ; Developmental Biology |
| 关键词[WOS] | CENTRAL-NERVOUS-SYSTEM ; EARLY MOUSE EMBRYOS ; RETINOIC-ACID ; NEURONAL DIFFERENTIATION ; IN-VITRO ; HOMEOBOX GENE ; CHICK-EMBRYO ; RADIAL GLIA ; TRANSCRIPTION FACTOR ; XENOPUS EMBRYOS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000251385500003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1598]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xia, CH,Wang, C,Zhang, KJ,et al. Induction of a high population of neural stem cells with anterior neuroectoderm characters from epiblast-like P19 embryonic carcinoma cells[J]. DIFFERENTIATION,2007,75(10):912-927. |
| APA | Xia, CH,Wang, C,Zhang, KJ,Qian, C,&Jing, NH.(2007).Induction of a high population of neural stem cells with anterior neuroectoderm characters from epiblast-like P19 embryonic carcinoma cells.DIFFERENTIATION,75(10),912-927. |
| MLA | Xia, CH,et al."Induction of a high population of neural stem cells with anterior neuroectoderm characters from epiblast-like P19 embryonic carcinoma cells".DIFFERENTIATION 75.10(2007):912-927. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。