The CCAAT-binding factor CBF/NF-Y regulates the human acetylcholinesterase promoter activity during calcium ionophore A23187-induced cell apoptosis
文献类型：期刊论文
| 作者 | Zhu, H; Gao, W; Shi, YF; Zhang, XJ |
| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
 |
| 出版日期 | 2007 |
| 卷号 | 1770期号:10页码:1475-1482 |
| 关键词 | acetylcholinesterase CBF/NF-Y promoter activity the distal CCAAT motif |
| 通讯作者 | Zhang, XJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 YueYang Rd, Shanghai 200031, Peoples R China.,xjzhang@sibs.ac.cn |
| 英文摘要 | We previously reported that the expression of acetylcholinesterase during A23187-induced apoptosis of HeLa cells is regulated by Ca2+ mobilization through the modulation of mRNA stability and acetylcholinesterase promoter activity. Transactivation of the human acetylcholinesterase promoter by A23187 was partially mediated by the distal CCAAT motif within the - 1270 to - 1248 fragment of the human acetylcholinesterase promoter, which was bound by the CCAAT binding factor (CBF/NF-Y). In the present study, we investigated the molecular mechanisms by which CBF/NF-Y regulates A23187-induced activation of the human acetylcholinesterase promoter. The results indicate that CBF/NF-Y binding to the distal CCAAT motif suppresses the promoter activity. Electrophoretic mobility shift assays (EMSAs) demonstrated that binding of CBF/NF-Y to the distal CCAAT motif decreased after A23187 treatment. Our results suggest that acetylcholinesterase promoter activation during A23187-induced HeLa cell apoptosis may result partly from the dissociation of CBF/NF-Y from the distal CCAAT motif in the acetylcholinesterase promoter, reversing this suppression. (c) 2007 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | FACTOR NF-Y ; TRANSCRIPTIONAL REGULATION ; MYOGENIC DIFFERENTIATION ; NEUROMUSCULAR-JUNCTIONS ; EXPRESSION ; GENE ; PROTEIN ; SUBUNIT ; INDUCTION ; MYOTUBES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000250187600006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1601]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhu, H,Gao, W,Shi, YF,et al. The CCAAT-binding factor CBF/NF-Y regulates the human acetylcholinesterase promoter activity during calcium ionophore A23187-induced cell apoptosis[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2007,1770(10):1475-1482. |
| APA | Zhu, H,Gao, W,Shi, YF,&Zhang, XJ.(2007).The CCAAT-binding factor CBF/NF-Y regulates the human acetylcholinesterase promoter activity during calcium ionophore A23187-induced cell apoptosis.BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,1770(10),1475-1482. |
| MLA | Zhu, H,et al."The CCAAT-binding factor CBF/NF-Y regulates the human acetylcholinesterase promoter activity during calcium ionophore A23187-induced cell apoptosis".BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1770.10(2007):1475-1482. |
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