Identification of six new box C/D snoRNA gene clusters from rice
文献类型：期刊论文
| 作者 | Li, W; Jiang, G; Zeng, DB; Jin, YX |
| 刊名 | IUBMB LIFE
 |
| 出版日期 | 2007 |
| 卷号 | 59期号:10页码:664-674 |
| 关键词 | 2 '-O-ribose methylation box C/D snoRNA rice plant |
| 通讯作者 | Jin, YX (reprint author), Chinese Acad Sci, Shanghai Inst Life Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,yxjin@sibs.ac.cn |
| 英文摘要 | Here we have identified 18 different box C/D snoRNAs encoded in six new gene clusters by the screening of Oryza sativa (rice) genome sequences using a computer program that we have designed to search for non-canonical box C and D sequences. Among this snoRNA collection, five are homologues of snoRNAs previously reported in other plants. One snoRNA is implicated to guide methylation at a corresponding site also modified in yeast rRNA but not in any other plants species yet examined. The other 12 snoRNAs are implicated in guiding 12 completely novel rRNA 2'-O-ribose methylation sites. We have verified the methylation of these rRNA sites by dNTP-concentration dependent primer extension assay. The expression of all snoRNAs reported here was experimentally confirmed by primer extension assay. Polycistronic precursor transcripts from the gene clusters have been demonstrated by RTPCR. Thus it is highly likely that each of the rice snoRNA gene clusters identified here is transcribed in a polycistronic way, regardless of whether or not they are encoded within introns. This indicates that the mode of polycistronic expression of snoRNA clusters is widespread in rice, as is the case in Arabidopsis thaliana. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | SMALL NUCLEOLAR RNAS ; NON-MESSENGER RNAS ; SMALL NUCLEAR RNAS ; RIBOSOMAL-RNA ; ORYZA-SATIVA ; ARABIDOPSIS-THALIANA ; 2'-O-RIBOSE METHYLATION ; GUIDE RNAS ; CANDIDATES ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000249643400006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1605]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, W,Jiang, G,Zeng, DB,et al. Identification of six new box C/D snoRNA gene clusters from rice[J]. IUBMB LIFE,2007,59(10):664-674. |
| APA | Li, W,Jiang, G,Zeng, DB,&Jin, YX.(2007).Identification of six new box C/D snoRNA gene clusters from rice.IUBMB LIFE,59(10),664-674. |
| MLA | Li, W,et al."Identification of six new box C/D snoRNA gene clusters from rice".IUBMB LIFE 59.10(2007):664-674. |
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