Identification of six novel T-1 conotoxins from Conus pulicarius by molecular cloning
文献类型：期刊论文
| 作者 | Peng, C; Wu, XC; Han, YH; Yuan, DD; Chi, CW; Wang, CG |
| 刊名 | PEPTIDES
 |
| 出版日期 | 2007 |
| 卷号 | 28期号:11页码:2116-2124 |
| 关键词 | cone snail Conus pulicarius T-1 conotoxin cDNA cloning sequence diversity |
| 通讯作者 | Wang, CG (reprint author), Tongji Univ, Coll Life Sci & Technol, Inst Prot Res, 1239 Siping Rd, Shanghai 200092, Peoples R China.,chunguangwang@mail.tongji.edu.cn |
| 英文摘要 | Cone snails are a group of ancient marine gastropods with highly sophisticated defense and prey strategies using conotoxins in their venom. Conotoxins are a diverse array of small peptides, mostly with multiple disulfide bridges. Using a TRACE approach, we identified six novel peptides from the venom ducts of a worm-hunting cone snail Conus pulicarius. These peptides are named Pu5.1-Pu5.6 as their primary structures show the typical pattern of T-1 conotoxin family, a large and diverse group of peptides widely distributed in venom ducts of all major feeding types of Conus. Except for the conserved signal peptide sequences in the precursors and unique arrangement of Cys residues (CC-CC) in mature domains, the six novel T-1 conotoxins show remarkable sequence diversity in their pro and mature regions and are, thus, likely to be functionally diversified. Here, we present a simple and fast strategy of gaining novel disulfide-rich conotoxins via molecular cloning and our detailed sequence analysis will pave the way for the future functional characterization of toxin-receptor interaction. (C) 2007 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Pharmacology & Pharmacy |
| 类目[WOS] | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy |
| 关键词[WOS] | T-SUPERFAMILY CONOTOXINS ; MASS-SPECTROMETRY ; VENOM PEPTIDES ; POSTTRANSLATIONAL MODIFICATIONS ; GAMMA-CARBOXYLATION ; CYSTEINE PATTERN ; NEUROPATHIC PAIN ; CHANNEL ; SNAILS ; DISCOVERY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000251104300004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1614]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Peng, C,Wu, XC,Han, YH,et al. Identification of six novel T-1 conotoxins from Conus pulicarius by molecular cloning[J]. PEPTIDES,2007,28(11):2116-2124. |
| APA | Peng, C,Wu, XC,Han, YH,Yuan, DD,Chi, CW,&Wang, CG.(2007).Identification of six novel T-1 conotoxins from Conus pulicarius by molecular cloning.PEPTIDES,28(11),2116-2124. |
| MLA | Peng, C,et al."Identification of six novel T-1 conotoxins from Conus pulicarius by molecular cloning".PEPTIDES 28.11(2007):2116-2124. |
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