Identification of candidate prostate cancer biomarkers in prostate needle biopsy specimens using proteomic analysis
文献类型：期刊论文
| 作者 | Lin, JF; Xu, J; Tian, HY; Gao, X; Chen, QX; Gu, Q; Xu, GJ; Song, JD; Zhao, FK |
| 刊名 | INTERNATIONAL JOURNAL OF CANCER
 |
| 出版日期 | 2007 |
| 卷号 | 121期号:12页码:2596-2605 |
| 关键词 | 2-DE mass spectrometry needle biopsy prostate cancer |
| 通讯作者 | Zhao, FK (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,g_q001@yahoo.com.cn ; fkzhao@sibs.ac.cn |
| 英文摘要 | Although serum prostate specific antigen (PSA) is a well-established diagnostic tool for prostate cancer (PCa) detection, the definitive diagnosis of PCa is based on the information contained in prostate needle biopsy (PNBX) specimens. To define the proteomic features of PNBX specimens to identify candidate biomarkers for PCa, PNBX specimens from patients with PCa or benign prostatic hyperplasia (BPH) were subjected to comparative proteomic analysis. 2-DE revealed that 52 protein spots exhibited statistically significantly changes among PCa and BPH groups. Interesting spots were identified by MALDI-TOF-MS/MS. The 2 most notable groups of proteins identified included latent androgen receptor coregulators [FLNA(7-15) and FKBP4] and enzymes involved in mitochondrial fatty acid P-oxidation (DCI and ECHS1). An imbalance in the expression of peroxiredoxin subtypes was noted in PCa specimens. Furthermore, different post-translationally modified isoforms of HSP27 and HSP70.1 were identified. Importantly, changes in FLNA(7-15), FKBP4, and PRDX4 expression were confirmed by immunoblot analyses. Our results suggest that a proteomics-based approach is useful for developing a more complete picture of the protein profile of PNBX specimen. The proteins identified by this approach may be useful molecular targets for PCa diagnostics and therapeutics. (c) 2007 Wiley-Liss, Inc. |
| 学科主题 | Oncology |
| 类目[WOS] | Oncology |
| 关键词[WOS] | HEAT-SHOCK PROTEINS ; ANDROGEN RECEPTOR ; PHYSIOLOGICAL-ROLE ; PEROXIREDOXIN-VI ; BETA-OXIDATION ; CARCINOMA ; FILAMIN ; EXPRESSION ; ANTIGEN ; APOPTOSIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000251109000003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1616]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Lin, JF,Xu, J,Tian, HY,et al. Identification of candidate prostate cancer biomarkers in prostate needle biopsy specimens using proteomic analysis[J]. INTERNATIONAL JOURNAL OF CANCER,2007,121(12):2596-2605. |
| APA | Lin, JF.,Xu, J.,Tian, HY.,Gao, X.,Chen, QX.,...&Zhao, FK.(2007).Identification of candidate prostate cancer biomarkers in prostate needle biopsy specimens using proteomic analysis.INTERNATIONAL JOURNAL OF CANCER,121(12),2596-2605. |
| MLA | Lin, JF,et al."Identification of candidate prostate cancer biomarkers in prostate needle biopsy specimens using proteomic analysis".INTERNATIONAL JOURNAL OF CANCER 121.12(2007):2596-2605. |
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