Identification of the gene imds-60 in mouse epididymis
文献类型：期刊论文
| 作者 | Zhou, X; Wang, J; Liu, Q; Shen, J; Zheng, M; Zhang, YL |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2007 |
| 卷号 | 39期号:12页码:939-946 |
| 关键词 | epididymis DNase I-like domain mouse |
| 通讯作者 | Zhang, YL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai Key Lab Mol Androl State Key Lab Mol B, Shanghai 200031, Peoples R China.,ylzhang@sibs.ac.cn |
| 英文摘要 | Sperm maturation, including the acquisition of motility and the full ability to fertilize oocyte, occurs during its transit through the dynamic environment of the epididymis. However, the roles of many genes involved in the process of sperm maturation still remain to be found. Based on an expressed sequence tag named imds-60, which was first found in uterus but is highly expressed in epididymis, the full-length cDNA sequence of imds-60 with a complete open reading frame was obtained in mouse epididymis by GenBank searching, polymerase chain reaction-based procedures, and 5'- and 3'-rapid amplification of cDNA ends. This protein was predicted to have an N-terminal signal peptide and a C-terminal DNase I-like domain with nine transmembrane motifs in the middle part of the protein. Northern blot analysis showed that the mRNA of imds-60 was highly expressed in epididymis but at a rather lower level in uterus, seminal vesicle gland, and stomach. Further study revealed that the mRNA of imds-60 is only expressed in corpus and cauda regions of epididymis, not in caput. It is regulated partially by androgen and peaked in male mice aged from 3 weeks to adult. The imds-60 protein might play an important role in cell communication during sperm maturation. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | I-LIKE ENDONUCLEASE ; DNASE ; CLONING ; EXPRESSION ; LOCALIZATION ; SPERMATOZOA ; PROTEINS ; REGION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000251666700004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1617]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhou, X,Wang, J,Liu, Q,et al. Identification of the gene imds-60 in mouse epididymis[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2007,39(12):939-946. |
| APA | Zhou, X,Wang, J,Liu, Q,Shen, J,Zheng, M,&Zhang, YL.(2007).Identification of the gene imds-60 in mouse epididymis.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,39(12),939-946. |
| MLA | Zhou, X,et al."Identification of the gene imds-60 in mouse epididymis".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 39.12(2007):939-946. |
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